16 



Directions for the Use of Cultures Supplied by the Bacteriological 

 Department of the Ontario Agricultural College. 



This bottle contains bacteria for inoculating seed. 



The culture is sent you with the understanding- that it is to be used 

 for experimental purposes and that you will use it as directed and report 

 to us your success or failure. Follow directions carefully. 



i. For every sixty pounds of seed to be treated, take one and one- 

 half pints of clean, cool water in a small pail. 



2. Pour some of the water into the bottle, shake the bottle thoroughly 

 and pour back the water into the pail. Repeat this six or eight times. 

 The jelly-like substance in the bottle will not dissolve, but may be broken 

 up with a clean stick and stirred in the water. 



3. Pour the water from the pail over the seed and mix thoroughly. 



4. Spread out the seed to dry in a clean place out of the sunshine. 



5. The seed will dry in an hour and may be planted in the usual 

 manner as soon as it is dry. 



6. Do not add water to the culture in the bottle until you are ready 

 to plant the seed. 



7. Some untreated seed should be planted for comparison, and it is 

 well to plant this first. 



8. After the seedlings are one month old, search for nodules, "little 

 bunches," on the roots. Examine for nodules again after three months. 

 During the season note number and size of nodules and vigor of plant 

 growth from treated and untreated seed. 



