NUTRIENT MEDIA. 27 



of bac-toriu-liulon broth. No sii;ii of disease a[)pi'urecl in any case, 

 although the phiiits wore kept under observation for several weeks. 

 This expininient was repeated several times with ne^^ativc results, 

 indicating- that this org^anisni is not a producer of disease in young 

 green onions. 



Jlaw j)i,e2)lant. — Stalks of raw pieplant were washed with corrosive 

 sublimate and then in distilled water. With a sterile knife the out- 

 side was removed and the stalks were then cut into slices about 2 cm. 

 thick and four placed in each petri dish. Two of each foui- were 

 inoculated with a 'J-t-hour-old beef-l)rotli culture of th(> calla germ. 

 In two cases only was there any growth, and this was very feeble, 

 resulting at the end of Hve days in a slight brown discoloration. The 

 experiment was repeated several times, but in all cases the growth 

 was very feeble and hardly perceptible. 



liavi r*^/Z'J«//(^— Cabbao-e heads were obtained from the market, the 

 outer leaves were pulled oH". and inoculations were made into the 

 stumps and leaves of several plants, using a iJ-l-hour-old beef-l)roth 

 culture of the calla germ, several heads })eing left for control. In 

 twenty-four hours the inoculated spots were slightly discoloi'cd. The 

 color- deepened for nine days (temperature, 18^ to 27"^ C), at the end 

 of which time the rot had spread over the whole surface of the stumps 

 and entirely through them. The color was Drab, No. IS, Plate III, 

 Ridgway, or somewhat darker than Saccardo's Avellaneus, No. 7, 

 Table I. At the same time the decay progressed in the leaves, pro- 

 ducing the same color and advancing from leaf to leaf until at the end 

 of nine days the whole of (>ach inoculated head was affected. None of 

 the control plants was aii'ected during this time. The decayed speci- 

 mens had the odor of rotten cabbage and in reaction were strongly 

 alkaline to litmus. 



In addition to these experiments with cabbage, pieces of stumps and 

 leaves were washed with corrosive sublimate, then with sterile water, 

 and placed in petri dishes, four pieces in each dish, two of which 

 were inmiediately inoculated with a- 2I:-hour-old beef-broth culture of 

 the organism and two left for control. In twentv-four hours at 20-^ 

 to 25^ C. the inoculated pieces began to show discoloration and in five 

 days the inoculated pieces w^ere decayed throughout. The control 

 pieces remained sound, except in a few instances in which the exuding 

 juice from the decayed pieces came into contact with the controls, in 

 which cases the latter decayed. The color, odor, and reaction were 

 the same as in the experiments with the whole heads, as previously 

 described. 



Haw 2)(irsnips. — Raw parsnips were obtained from the market and 

 treated in the same way as the raw potatoes. With a sterile knife 

 pieces of convenient thickness were cut and placed in sterile petri 

 dishes, four pieces in each dish. Two pieces in each dish were inocu- 



