28 A SOFT ROT OF THE CALLA LILY. 



latcd with the calUi-rot germ, using a 24- hour-old heef- broth culture. 

 At the end of twenty-four hours after inoculation the inoculated 

 pieces began to show discoloration at the points of infection, and at 

 the end of three days (temperature, 18° to 25^ C.) the discoloration 

 was very marked (PI. VII, fig. 1). The inoculated pieces had a pun- 

 gent, sweetish odor and were plainly alkaline to litmus. The color 

 corresponded to Ridgway's ^Vlummy Brown, No. 10, Plate III, or nearly 

 to Saccardo's Fuligineu.s, No. 11, Table I. 



Rain 6'«/7y>j5.s.— Several roots of carrots were obtained from the 

 market and prepared in the manner indicated above. Slices of suit- 

 able thickness to be placed in petri dishes were then cut off with a 

 sterile knife. Four pieces were placed in each petri dish, and as in 

 the other experiments two out of each set were inoculated with the 

 calla-rot organism and two left for control. In twenty-four hours 

 at 20° to 22° C. the inoculated pieces began to discolor at the points 

 of inoculation, and in three days the discoloration was very striking 

 over the entire surface of the inoculated pieces (PI. VII, fig. 2). In 

 the central part of the root the discoloration had extended entirely 

 through, a distance of 2 cm., while toward the outer surface the 

 progress w^as not so rapid, the discoloration having extended only 

 about 1 cm. The color of the inoculated pieces three day;^ after inoc- 

 ulation was Vandyke Brown, No. 5, Ridgway's Plate III, or nearly 

 Saccardo's Fuligineus, No. 11, Table I. The decayed part was dis- 

 tinctly alkaline to litmus. At the end of eight days the inoculated 

 pieces were entirely discolored and soft, while the uninoculated pieces 

 still retained their normal color and were sound. At this time the 

 inoculated pieces had changed in color from Vandyke Brown or Fulig- 

 ineus to Olive, No. 9, Ridgway's Plate III, or to Saccardo's Oliva- 

 ceus. No. 39, Table II. 



Raio turnips. — A firm, white turnip was obtained from the market, 

 prepared for the petri dishes, and inoculated in the same manner as 

 the other vegetables. In twenty-four hours discoloration was dis- 

 tinctly noticeable at the points of inoculation, and in three days the 

 discoloration was very striking and had progressed downward from 2 

 to 3 mm., while the uninoculated pieces were still white and sound 

 (see PI. VllI, fig. 1). The color of the inoculated pieces at this time 

 closely resembled Ridgway's Olive, No. 9, Plate III, or Saccardo's 

 Olivaceus, No. 39, Table II. The discolored parts were strongly 

 alkaline to litmus and had a striking odor of decayed turnips. 



liav) salsify. — Several roots of salsify were obtained from the mar- 

 ket and the same method was used in preparing and inoculating them 

 that was employed with the other vegetables. In twenty-four hours 

 the inoculated pieces were discolored and in three days all had discol- 

 ored but only the inoculated pieces had decayed, and as these kept 

 their shape it was impossible to bring out the difference in color by 



