EFFECT OF ('(HM'KR I'l'oX rATlKKJKNIC 1?AC'TEKIA. 



31 



Effect of copjitr huIjiIkiIc n/ion littrillus tiiphi at ronm temperatun." 

 [Dt'termiiialiDii made in I'ctri di.shf.s.] 



Diinitioii fif oxposiiro 

 to Hctiiiu of i-opjior Check, 

 sulpha te. 



One part copper sulphate to— 



100,000 parts 200.000 parts | 500,000 parts 

 water. water. water. 



i hour.. 



1 hour. . 

 U hour>s 



2 hourfi. 

 2i hours 



3 hours. 

 3i hours 



4 hours. 

 8 hours. 

 12 hours 



1 . IWjO 

 1,S30 

 1 , rm 

 1 , l«o 



yyj 



1,134 



1,0«0 



7X3 



270 



Colunun. 



5, -181 



Cutonk*. 

 •>, 370 



UlS 



l,02li 



86-1 



243 



IKO 



1S6 



108 







l> 



-MOtj 



1,242 ! 



l.JW". 



1,(120 



1,101 



783 



972 



72 



14 



t\AouUi>. 

 2, 7.'i4 

 2, 403 

 1,323 



2, S3.'> 



1,4H.T 



1,620 

 'J18 



1,998 

 4C5 



1,000,000 parte 

 water. 



5,000,000 parts 

 water. 



CulimicK. 

 2, <M(i 

 1,377 

 2, 673 

 2, 430 

 2, 727 

 1,782 

 2, 079 

 1,836 

 324 

 243 



Oulutiifg. 



3,645 



1,755 



2,808 



3,024 



2,106 



756 



1,242 



1,458 



459 



405 



" E.\pcrimei)t conducted in test tubes each contaiulng 6 ec. of sterilized water, jtortions of which 

 hiKl been previously treated with the desired uniount.s of copper sulphate. All tubes inoculated with 

 a 3 mm. loop of an "l«-hour culture of Jl. Ii/plii. 



Effect of copper sulplmte upon Bacillus typhi at room temperature.'^ 

 [Determination made in Petri dishes.] 



Durati(m of expo- 

 sure to action of 

 copper sulphate. 



hour ... 



3 hours . . 



4 hours . , 

 C hours . . 

 8 hours . . 

 12 hours . 



No. I. Check. 



Colonies. 





14, 

 116, 



'(24, 

 [19, 



'p, 

 119, 

 j6, 

 l21, 



144 



792 

 6C4 

 212 

 954 

 558 

 300 

 400 

 484 

 074 

 156 

 600 



5 

 4 



7 

 

 2 



31 



8 

 

 U 

 

 33 

 



No. 2. One part ' No. 3. One part 

 copper sul- ci)p|>er sul- 

 phate to phate to 

 200,000 parts 100,000 parts 

 water. water. 



' No. 4. One j>art 

 copper sulpiiate 

 to 50,000 parts 

 water. 



Colonies. 



n 



108 

 90 

 11 



126 

 

 

 

 

 

 

 

 



a. 



Colonies. 



56 — 



si 



n 



3 

 198 

 72 

 6 

 

 

 

 

 

 

 

 





c» 



Col()nie.s. 





No. 5. One part 

 copper sul- 



])liate to 



100.000 part-s 



water. 



Colonies. 



n 



234 

 306 

 6 

 4 

 

 

 

 

 

 

 

 



5.S 



CO 



aExperiment conducted in 12-liter aquaria. No. 1 was untreated: copper .sulphate was added to 

 Nos. 2, 3, -4. and ft. Three cubic centimeters of a mixture of ctiltures of Jl. ti/phi were added to each 

 jar 18 hours before treating. All small nonliquifyiug colonies counted as typhoid. 



28480— No. 64—04 3 



