8 CONTENTS. 



Page. 

 Description of Bacterium tumefaciens from daisy — Continued. 

 Morphological characters — Continued. 



Capsules 107 



Zoogloeae 107 



Involution forms 107 



Behavior toward stains 107 



Cultural characters 108 



Nutrient agar 108 



Corn-meal agar 109 



Potato 109 



Starch jelly 110 



Nutrient gelatin 110 



Loeffler's blood serum Ill 



Nutrient beef broth Ill 



Alkaline beef broths 112 



Sugared peptone water 112 



MUk.... 112 



Litmus milk 113 



Silicate jelly 113 



Cohn's solution 113 



Uschinsky 's solution 114 



Sodium chloride bouillon 114 



Growth in bouillon over chloroform 114 



Nitrogen nutrition 114 



Best media for long continued growth 115 



Quick tests for differential purposes 115 



Fermentation tubes 115 



Ammonia production 116 



Nitrates 116 



Indol 116 



Toleration of acids 117 



Toleration of sodium hydroxide 117 



Optimum reaction for growth in bouillon 118 



Vitality on culture media 120 



Temperatiire relations 120 



Thermal death point 120 



Optimum temperature 120 



Maximum temperatui'e 121 



Minimum temperature 122 



Effect of drying 123 



Effect of sunlight 124 



Acids 125 



Alkalies 125 



Alcohols 125 



Ferments 125 



Crystals 125 



Effect of germicides 125 



Pathogenicity 126 



Loss of virulence 126 



Group number 126 



Immunity 127 



213 



