10 CONTENTS. 



Cultural characters— Continued. Page. 



Reduction of nitrates— Continued. 



Quince • ^^^ 



Poplar 152 



Remarks 1^2 



Growth in bouillon over chloroform 152 



Inversion of cane sugar 152 



Nitrogen nutrition — Table X 153 



Experiments with litmus milk 154 



Silicate jelly 156 



Inoculable and crossinoculable 156 



Discussion of question of species, varieties, and races of the crown-gall organism . 157 



Local reaction of the inoculated plant 158 



Young versus old tissues 158 



Structure and growth of the tumor 159 



Suggested relationship to animal tumors 161 



Metastases 171 



Chemical changes 173 



Excess of oxydizing enzymes in the gall tissue 173 



Other changes in the tissues 173 



Analysis of flask cultiu-es of Bacterian tumefaciens 174 



The stimulus to growth 175 



Physical changes; early decay 175 



Effects of the disease on the tissues not directly involved 176 



Physical effects 176 



Physiological effects 176 



Experiments showing increased resistance of the host due to repeated inocula- 

 tions and also decreased virulence of the bacteria 177 



Losses due to crown gall 183 



The daisy 183 



The almond, the peach, and other stone fruits 183 



Apple trees 185 



The quince 188 



The raspberry and the blackberry 188 



The rose 189 



The grape 190 



Red clover 191 



Alfalfa .' 191 



Cotton 191 



Hops 191 



Sugar beets 191 



Tuberculosis of beets 194 



Description of Bacterium beticolum n. sp 194 



Shrubs, shade trees, and forest trees 195 



Hothouse plants 196 



Best method of dealing with the disease 196 



Synopsis of conclusions respecting crown gall 197 



Index 203 



213 



