28 CROWN-GALL OF PLANTS. 



Result— March. 30, 1908: Galls had formed on all the inoculated 

 plants. The checks did not contract the disease. 



DAISY ON FIELD DAISY. 



Inoculations of April 15, 1907 (Brown). 



Wild oxeye daisy plants {Chrysanthemum leucanthemum var. pin- 

 natijidum) transferred from a field near Washington and grown in 

 pots in the greenhouse, were inoculated on the young stems and also 

 on the leaves. Four plants were inoculated in 4 or 5 places on each, 

 and check pricks were made on one plant. 



Result. — April 22, 1907: Galls had formed on all of the inoculated 

 stems at the places pricked. None appeared on the inoculated 

 leaves. 



May 11, 1907: The numerous galls did not grow to the size of those 

 on the cultivated daisy, the largest ones being only half an inch in 

 diameter. The check plant did not contract the disease. 



DAISY ON JAPANESE CHRYSANTHEMUM. 

 Inoculations op May 6, 1907 (Brown). 



Three hothouse chrysanthemum plants were inoculated by needle 

 pricks on the stems and leaves with agar streak cultures 2 days old. 

 Check punctures were made on two other plants. 



Result. — July 19, 1907: Galls had formed at all points of inocula- 

 tion on the stems; none appeared on the leaves. The checks bore 

 no galls. 



daisy on chrysanthemum coronarium and on shasta daisy 



(burbank hybrid). 



Inoculations of July 23, 1907 (Brown). 



Six plants of Chrysanthemum coronarium, and 6 of Shasta daisy 

 were inoculated by needle pricks on the lower parts of the stems 

 with agar streak cultures 5 days old. Checks on both plants were 

 held. 



Result. — August 27, 1907: Galls had formed on all the inoculated 

 plants at the places punctured. Those on the Shasta daisy were 

 quite large. The checks remained free. 



September 30, 1907: The inoculated Chrysanthemum coronarium 

 are all dead, apparently as a result of the inoculation. 



DAISY ON THE CORN MARIGOLD. 



Inoculations of August 1, 1907 (Brown). 



Six plants of the corn marigold (Chrysanthemum segeium) were 

 inoculated on the stems by needle pricks with agar streak cultures 2 

 days old ; two checks were held. 



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