34 CROWN-GALL OF PLANTS. 



Inoculations of March 12, 1908 (Smith). 



Five olive plants were inoculated with the daisy organism from 

 agar streak cultures 48 hours old in soft wood near the growing tip 

 of each shoot. They were held as checks on the second set of oleander 

 inoculations (a set which produced tumors). Five daisy plants were 

 inoculated from the same cultures for controls. 



Result. — May 21, 1908: The results by plants on this date are as 

 follows : 



Plant L: Terminal shoot has grown only 2 inches beyond the 15 needle pricks; no 

 tumors. 



Plant M: Terminal shoot has grown vigorously a distance of about a foot beyond the 

 pricked portion; about 30 needle pricks; no tumors. 



Plant N: Terminal shoot has 20 needle punctures, no tumors; slightly raised rough 

 corky places where the pricks have healed. Shoot has grown vigorously a foot beyond 

 the needle pricks. 



Plant 0: Terminal shoot has about a dozen punctures, slight corky projections 

 where needle entered. These raised portions are perhaps one-third millimeter in 

 diameter and the healed corky portion of the wound itself possibly 1 mm. in diameter. 

 All are alike on this and on the other plants. There are no tumors. The terminal 

 shoot has grown over a foot beyond the point of inoculation. 



Plant P: Basal shoot and terminal shoot inoculated; about 12 needle punctures. 

 Shoots have grown about 6 inches beyond the pricks; no tumors. 



November 16, 1908: No tumors. 



The daisy plants inoculated at the same time all produced good- 

 sized tumors promptly. 



DAISY ON VEGETABLES (bEET, RADISH, CARROT, ETC.). 

 Inoculations of April 26, 1907 (Smith and Brown). 



The vegetables were purchased at the market and taken to the 

 laboratory, where they were washed thoroughly and inoculated. 

 Thirty punctures, in groups of 5, were made on both checks and 

 inoculated plants. Agar streak cultures 2 days old were used for 

 the inoculations. The varieties and numbers of plants used were: 

 Radish, long and round mixed, 10; turnips, 5; rutabagas, 2; par- 

 snips, 6; carrots, 4; red beets, 3. Two to four checks were held of 

 each variety, except the rutabagas. 



Result. — June 28, 1907: A radish plant with a large irregular tumor 

 on one side of the root was brought in on this date and photographed. 



July 19, 1907: The rutabagas, parsnips, and round radishes were 

 dead — i. e., they did not grow. Galls had formed on the inoculated 

 beets, long radishes, and carrots. On the radishes they were 1 inch 

 to 1 h inches in diameter. Small galls one-fourth inch across were on 

 all red beets except one, which was about Ih inches across. Small 

 galls not larger than a half inch were on the carrots. The checks 

 were free. 



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