36 CROWN-GALL OF PLANTS. 



Result. — June 27, 1907: The plant died. It was pulled up and no 

 tumor found on the stem. 



Inoculations of August 9, 1907 (Brown). 



Eight plants were inoculated by needle pricks on the youngest 

 parts of the stems with agar streak cultures 3 days old. Three checks 

 were made. The varieties used were Prince Albert, Black Prince, 

 and White Tokay. The vines were 2 feet tall and had made rapid 

 growth. 



Result. — August 27, 1907: Small knots had formed on all but 2 

 plants. Of the latter, 1 was a White Tokay and the other was a 

 Black Prince. No knots formed on the checks. 



Inoculations op March 7, 1908 (Smith). 



Three plants of Vitis vinifera, 1 each of varieties Prince Albert, 

 Barnes Muscat, and Black Prince, were inoculated by needle pricks 

 in two places on each with 48-hour agar slants of the daisy organism. 



Result. — One of the 3 developed a small tumor. The plants made 

 very little growth. 



DAISY ON AMERICAN GRAPES. 



Inoculations op April 3, 1907 (Smith and Brown). 



Four varieties of grape were inoculated with 2-day-old agar streak 

 cultures. Thirty punctures were made on each in groups of 10 — 

 on the root, on the underground stem, and at the base of young shoots 

 near the top of the stem. Two plants of each variety were inocu- 

 lated and 1 of each held as a check, the check receiving the same 

 number of pricks from a sterile needle. The varieties were as follows: 

 Moore Early, Delaware, Concord, Martha. 



Result. — May 9, 1907: A small gall was found on the underground 

 stem of a Martha grapevine. No galls found on the others. All were 

 dormant when inoculated. 



DAISY ON IMPATIENS. 



Inoculations of May 26, 1908 (Smith). 



One young growing plant of Impatiens sultani was inoculated from 

 an agar streak culture 4 days old. The plant was punctured on the 

 soft stem in several places. A daisy plant inoculated from the same 

 culture was held for control. 



Result. — No galls resulted. The plant was under observation for 

 several months. No record respecting the control. 



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