EXPERIMENTS WITH THE DAISY ORGANISM. 43 



Remarks. — No positive conclusions can be drawn from this experi- 

 ment, since at least some of the trees came from infected soil, as 

 shown by the hairy root. 



Inoculations of May 26, 1908 (Sihth). 



Five vigorous shoots of Wealthy apple (in the hothouse) were 

 inoculated near the tip with the daisy organism from an agar streak 

 4 days old. As a check on these a daisy plant was inoculated in 

 two places from the same tube used to inoculate the apple shoots. 



Result. — June 1, 1908: The shoots are vigorous — about 3 feet long. 

 The daisy is developing tumors in both the places inoculated. Apple 

 tumors, therefore, should be obtained later. Nothing definite now. 



September, 1909: No tumors appeared on the apples. 



Inoculations of July 20, 1910 (Smith and Brown). 



Trees 1 and 2 years old were inoculated on shoots and on crowns 

 by needle pricks from young agar cultures of a recent isolation. 



Result. — October 22, 1910: Negative above ground and uncertain 

 below. Numerous galls were present on the crowoi and roots of a 

 number of the trees, but Schizoneura lanigera was present. 



DAISY ON ROSE. 



Inoculations op March 27, 1907 (Brown). 



Very young rose shoots were inoculated by needle pricks with 

 slant agar cultures 2 days old. The shoots of 3 plants were punc- 

 tured with a sterile needle for checks. 



Result. — April 19, 1907: Small knobbed protuberances were formed 

 at each inoculated place; the checks were free from knobs. 



Inoculations of April 3, 1907 (Smith and Brown). 



Eighteen rosebushes were inoculated with agar streak cultures 2 

 days old. The plants, including the 6 healthy checks, were washed 

 thoroughly in running water. The varieties were Bridesmaid and 

 Bride. Nine plants of each variety were inoculated and 3 checks 

 held of each variety. Each plant received 10 to 20 punctures. 

 Some were inoculated at the base of the shoot on the main stem, 

 some on the stem below ground, and some at the base of young 

 shoots. All were growing slowly. The plants were in pots in the 

 greenliouse. Daisy plants were inoculated for control. 



Result. — May 9, 1907: No galls on the rosebushes. The daisy 

 controls developed galls. 



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