EXPERIMENTS WITH THE DAISY ORGANISM. 45 



Inoculations of April 18, 1907 (Smith and Brown). 



Two cabbage plants were inoculated on the midribs of a dozen 

 outer leaves; checks were held on another plant. 



Result. — April 26, 1907: Each one of the inoculated midribs had 

 split, and on these splits were knobbed outgrowths. The checks 

 remained free from splits and knobs. 



June 28, 1907: A cabbage stalk brought from the hothouse showed 

 numerous tumors growing out of the leaf scars, the lower (inoculated) 

 leaves having fallen. These appear to be secondary infections. 

 Young sprouts are growing out of the tumors. Several other plants 

 (none of which were inoculated on the stems, but all on the leaves) 

 at this time showed similar tumors growing out of the leaf scars.** 



Inoculations of March 7, 1908 (Smith). 



Three leaf scars on each of three cabbage plants were inoculated 

 with 48-hour agar slants of the daisy organism. 

 Result. — Nothing definite. 



DAISY ON CARNATION. 



Inoculations of March 2, 1907 (Townsend). 



Twelve inoculations were made into stems of carnation {Bianthus 

 can/0 pTiyllus), using agar streak cultures of February 27. Ten of 

 these inoculations were made near the growing tips and two about 

 midway of the older stems. Six controls were made in the same 

 relative positions on other plants. 



Result. — Knots or galls formed in all cases at the points of inocula- 

 tion in times varying from two to six weeks. No galls formed on 

 the controls. One of the galls was photographed September 18 

 (PI. VII, fig. 1). 



DAISY ON SUGAR BEET. 



Inoculations of April 17, 1907 (Brown). 



A row of young sugar beets about 4 inches high growing in the 

 greenhouse was used for these inoculations. The soil was turned 

 back from the root, the root washed with sterile water and inoculated 

 with agar streak cultures 2 days old; the punctured places were cov- 

 ered with moist cotton, and the soil replaced. Twelve plants were 

 treated m tliis way, and three were punctured with a sterile needle 

 for checks. 



o The senior writer secured infections in the laboratory on the freshly cut surface of raw turnip roots 

 kept in deep sterile Petri dishes. The bacteria were rubbed on the surface with a platinum loop after the 

 root had been scrubbed, soaked for an hour in mercuric chloride water (1:1,0001 and cut with a sterile knife 

 (PI. IX, fig. 2). \ yellow turnip bearing galls not due to nematodes was received from Texas. 

 213 



