EXPERIMENTS WITH THE DAISY OEGANISM. 49 



No. 181: A tumor on the root about 6 inchea from the top; not larger than a small pea. 



No. 182: Two tumors on the upper part of the root, each about a half inch in diame- 

 ter; also a small tumor on a side root not larger than a sweet-pea seed. 



No. 183: Plant missing. 



No. 184: Two small tumors on the roots underground; one is not larger than a small 

 pea and the other a little smaller. 



No. 185: Four tumors on the upper part of the roots; 1 is three-fourths inch in 

 diameter; another, a small tumor, is on the base of a young shoot which is tumefied 

 over a distance of 2 inches toward the base, and it is probable that the organism had 

 to do with this tumefaction. Saved separately in alcohol for sections. 



No. 186: This is a defective, slow-growing root; it has a tumor about as large as a 

 pea on the main root 4 inches from the top. 



No. 187: Two tumors on the upper part of the root where stems come out and 1 just 

 above on another stem; each half an inch or more in diameter. 



Remarks. — Eighteen plants, all diseased; jar of material saved in 

 alcohol; photographs made; 8 check plants subsequently examined — 

 all free. These check plants received more than 200 punctures. 



Inoculations of April 10, 1907 (Brown). 



Two more varieties of hops were inoculated, the Red Canada and 

 the Humi:)hrey. The plants were left in the pots but the soil was 

 turned back and the crown and young shoots were washed with 

 sterile water. Eighteen of the Red Canada variety were inoculated, 

 9 with agar streak cultures 4 days old, and 9 with cultures 6 days old. 

 Seven of the Humphrey were inoculated with a 6-day-old culture. 

 Twenty to 30 needle pricks were made in the base of young shoots 

 and in the crown. Two checks of each variety were held, the same 

 number of pricks being made with a sterile needle. 



Result.— A.^T\\ 27, 1907: All the 25 inoculated plants had galls 

 except one, which was dead. Most of the galls were on the crowTi; 

 only a few occurred at the base of the shoots. The 4 checks had 

 none. 



July 25, 1907: Removed the last of the inoculated hops (PI. IX, 

 fig. 1). The galls were tliree-fourths inch to IJ inches in diameter. 

 The checks remained free. 



RemarTcs. — Of the 43 inoculated hops, 42 contracted the disease, 

 or 100 per cent, if we exclude the 1 feeble plant which died soon 

 after inoculation. Of the 12 checks, all remained free from the 

 disease. 



DAISY ON FIG. 



Inoculations op August 9, 1907 (Brown). 



Eleven young trees of Ficus carica were inoculated by needle 

 pricks with agar streak cultures 3 days old. The plants were grown 

 from cuttings and were about a foot high. The youngest and softest 

 parts of the stems were inoculated. Three checks were held. 

 78026°— Bull. 213—11—4 



