50 CROWN-GALL OF PLANTS. 



Result. — August 27, 1907: No galls had formed. (None formed 

 later.) 



Inoculations op March 7, 1908 (Smith). 



Three fig plants were inoculated in the young tender tissues of the 

 growing part of the stem from 48-hour agar slants. 

 Result. — June 1, 1908: No tumors have developed. 

 September, 1909: No galls appeared. 



Inoculations of April 6, 1910 (Brown). 



Twelve young rapidly growing shoots of an edible fig were selected 

 and the inocuhitions made by needle pricks from a young agar 

 streak culture of the newest isolation from daisy. 



Result. — ^June 25, 1910: All negative. 



DAISY ON CHESTNUT. 



Inoculations of March 7, 1908 (Smith). 



Three Paragon chestnut plants and 1 American chestnut were 

 inoculated with 48-hour agar slants of the daisy organism. The 

 needle pricks were made on growing shoots. 



Result. — September, 1909: No galls appeared. 



Inoculations of April 7, 1910 (Brown). 



The newest isolation from daisy was employed, 4 of the chestnut 

 plants being inoculated on the crown and 6 on young shoots. All 

 were needle-prick inoculations from young agar streaks. 



Result. — June 25, 1910: All negative. The shoots grew rather 

 slowly. 



DAISY ON OAK. 



Inoculations op April 7, 1910 (Brown). 



Eight small seedling red oaks (species ?) were inoculated by needle 

 pricks into terminal slow-growing green shoots. 



Result. — ^June 24, 1910: All negative. The shoots made only an 

 inch of growth beyond the pricks. 



DAISY ON PERSIAN WALNUT. 



Inoculations op April 17, 1907 (Smith and Brown). 



The roots of 6 trees of Persian walnut (Juglans regia) were inocu- 

 lated with agar slants 5 da3^s old, each receiving 30 pricks in groups 

 of 5. Four trees were held as checks. The roots of all the trees 

 were washed thoroughly in the laboratory before inoculating. The 

 trees were then planted in the greenhouse. 



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