54 CKOWN-GALL OF PLANTS. 



Inoculations of May 7, 1910 (Brown). 



Six terminal young flower shoots of slow-growing old daisy plants 

 already bearing daisy galls on the main stem were selected for inocu- 

 lation. Into these the Arbutus organism was pricked from young 

 agar streaks. 



Result. — June 25, 1910: All negative. 



July 7: Same. 



ARBUTUS ON SUGAR BEET, 

 Inoculations of November 8, 1909 (Brown). 



Sixteen young sugar beets growing in the open bed were inoculated 

 with 2-day-old agar cultures of the Arbutus gall organism. Six 

 checks were held. 



Result. — December 20, 1909: Examined one row and found a gall 

 three-fourths of an inch in diameter on one beet only. It was left to 

 grow. 



March 26, 1910: Reexamined plant with gall and found that it had 

 rotted off. 



June 10, 1910: Pulled up the beets and found one other with a gall 

 2 inches in diameter. Much of it had rotted off, but new parts were 

 forming. This was photographed (PI. XXIV, A). 



COTTON ON DAISY. 



Inoculations of November 16, 1909 (Brown). 



A dozen inoculations were made on young stems of plants in fine 

 condition by needle pricks from agar cultures 5 days old. Three 

 checks were held. 



Result. — January 4, 1910: No galls formed. 



Inoculations of April 29, 1910 (Brown). 



Seven young shoots of the Queen Alexandra daisy were inoculated 

 with 2-day-old agar cultures of the cotton-gall organism, using needle 

 pricks. These plants were old but were putting out young flower 

 shoots. They already bore large galls on the lower part of the stem 

 as the result of earher inoculations with daisy. 



Result. — July 7, 1910: No galls resulted, 



COTTON ON COTTON, 



Inoculations of December 1, 1909 (Brown). 



Nine seedhng cotton plants about 6 inches tall were inoculated with 

 2-day-old cultures of the cotton-gall organism." The soil was laid 



« When this organism was originally isolated there were pure cultures on 6 of the 8 plates poured. 

 213 



