58 CEOWN-GALL. OF PLANTS. 



(4) Poured plates from last bouillon transfer made a few days before the beets were 

 inoculated. 



(5) Agar subcultures from colonies on 4. 



(6) Beets inoculated from one of 5. 



ALFALFA ON DAISY. 



Inoculations of June 14, 1909 (Brown). 



Four young daisy shoots were inoculated with the alfalfa gall 

 organism, using pure cultures 4 days old. 



Result. — August 20, 1909: One of the shoots inoculated had a 

 small gall about three-fourths of an inch in diameter. No good daisy 

 plants were available at this time, and the experiment was never 

 repeated. 



ALFALFA ON ALFALFA. 



Inoculations of June 14, 1909 (Brown). 



Four young alfalfa cuttings were inoculated with 4-day-old agar 

 streak cultures, the colonies for which were isolated June 1, 1909, 

 from an alfalfa gall occurring in a field in Alabama. The fine roots 

 were inoculated and then tied with a piece of cord, which was later 

 replaced by a wire. This was to locate the galls should they form, 

 and also so as not to mistake nitrogen-fixing nodules for them. 



Result. — July 6, 1909: The plants were knocked out of the pots 

 and examined. No galls were found. 



August 20, 1909: Examined plants again and found the inoculations 

 had taken on two-thirds of the roots marked by the wires. There 

 were no distinct galls like the daisy gall, but hairlike projections on 

 which a nodule different from the nitrogen-fixing nodule was formed 

 and from which fine roots projected. 



Inoculations of June 16, 1909 (Brown). 



Inoculated 4 roots on each of 2 alfalfa plants with colonies on a 

 plate poured June 10 (second isolation from southern alfalfa plants). 

 These plants were old and pot-bound, but were taken from pots and 

 repotted after inoculation. Each root was tied with a knot of 

 strong cord. 



Result. — July 6, 1909: Examined roots and found no galls. 



August 20, 1909: Concluded plants were too old and had made 

 too little growth for development of galls. , 



Inoculations op July 16, 1909 (Brown). 

 « 



Forty-eight seedling alfalfa plants were inoculated in the crown, 



in the roots, and also in the nitrogen-fixing nodules with 4-day-old 



agar cultures from second southern isolation, making 10 to 20 pricks 



in each plant. Twelve checks were held. 



213 



