78 CROWN-GALL OF PLANTS. 



Result. — December 22, 1908 : The 7 inoculated plants were examined 

 carefully and but 1 gall found (PI. II, fig. 6). It was three-fourths 

 inch in diameter and regular in shape. The checks were examined 

 and no galls found on them. 



RASPBERRY ON DAISY. 



Inoculations of July 9, 1907 (Smith). 



Four daisy plants were inoculated from the white colonies on 

 Petri-dish poured plates of July 2. These colonies were plated out 

 of a small growing tumor on the root of a red raspberry plant taken 

 from one of our houses, the same being in all probability a natural 

 infection. Most of the daisy plants inoculated had several branches, 

 and each branch was inoculated toward the top in as soft tissue as 

 could be found. The plants had been neglected, and the wood was 

 rather hard for the purposes of inoculation. 



Result. — Negative. 



QUINCE ON DAISl. 



Isolation or Organisms. 



The galls on quince trees are very unlike those of apple, peach, 

 chestnut, etc. — i. e., they are warty outgrowths massed together, 

 rather than galls of the ordinary type. 



Isolations were undertaken December 23, 1908. 



The material for this work was sent from Cahfornia by Mr. Bal- 

 lard, and when it arrived it was very much dried out. The galled 

 stems were soaked overnight and the plates poured from the softest 

 part of the material after proper scrubbing and surface sterihzation. 

 Six days afterward gall colonies appeared on the plates. 



Inoculations of January 27, 1909 (Brown). 



Inoculations were made into the stems of 8 daisy plants of both 

 yellow and white varieties (4 of each) with agar streak cultures 2 

 days old, the first subculture from poured-plate colonies. Three 

 checks of the white variety were held. All were old plants growing 

 slowly. 



Result. — February 11, 1909: wSmall laiobs were present on 2 of 

 the white daisies at the point of inoculation, but no indication of any 

 on the yellow variety. There were no outgrowths on the check 

 plants. 



Inoculations of March 21, 1909 (Brown). 



Several daisy plants of the Queen Alexandra variety were inocu- 

 lated by needle pricks with agar streak cultures 5 days old. Nine 

 different shoots were punctured. Two plants were also held as 

 checks, several shoots being punctured. 



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