EXPERIMENTS WITH THE QUINCE ORGANISM. 79 



Result. — April 3, 1909: Gall formation had begun to show on 

 two-thirds of the inoculated places. The appearance was rather 

 warty and not like the beginning of an apple or peach gall. The 

 checks remained free, 



QUINCE ON QUINCE. 



Inoculations of May 14, 1909 (Brown). 



Rooted cuttings of quince, from which the leaves had been pulled 

 off and the stems trimmed back, were scrubbed well and then inoc- 

 ulated. The stem and the part of the stem from which roots were 

 growing (it could scarcely be called crown on these cuttings) were 

 inoculated. Both nodes and internodes on the upper stem were 

 inoculated. From 30 to 50 punctures were made on each cutting. 

 The side inoculated was indicated by a notch in the bark. Eight 

 cuttings were inoculated and 6 checks were held. 



Result. — June 15, 1909: No indication of galls. 



July 13: Still no galls. 



November 28, 1910: All negative. 



Inoculations of May 21, 1909 (Brown). 



Some of the lot of cuttings received May 14, 1909, had been planted 

 without inoculating and were now starting to send out buds. Three 

 trees were inoculated on the stems by needle pricks with 2-day-old 

 agar cultures. Nodes where leaf buds were starting and also inter- 

 nodes were inoculated. Each stem received at least 30 punctures. 

 The same number of trees were inoculated with the hairy-root 

 organism in the same manner. (See Hairy root on quince trees, 

 p. 103.) 



Result. — September 3, 1909: Examined the trees and found no 

 trace of galls. The quince trees in the greenhouse seemed to grow 

 very little, for no change had taken place in the size of the young 

 stems inoculated. 



November 28, 1910 : Still no galls. Trees grew more than last year. 



Inoculations of March 9, 1910 (Brown). 



Reinoculated 7 of the quince trees which were inoculated last 

 May. They seemed to be in a good growing condition. Two-day- 

 old agar cultures of the quince-gall organism were used and only 

 young shoots were punctured — 6 to 8 shoots on each plant. 



Result. — June 24, 1910: No galls formed. 



November 28, 1910: Nothing. 



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