EXPERIMENTS WITH THE CHESTNUT ORGANISM. 91 



CHESTNUT ON GRAPE. 

 Inoculations of Junk 10, 1!)I0 (Brown). 



In 1910 a fresh strain of the chestnut gail organism was plated 

 from an old gall growing on the crown of a young chestnut (material 

 from ^Ir. Davitl Fairchild). A single shoot of Vitis vinifera was 

 inoculated by needle pricks from a subculture on agar. 



Besult. — June 24, 1910: Distinct small knobs are visible on some 

 of the needle pricks. Altogether about a dozen are now visible. 



October 31, 1910: Slight elevations in the needle pricks, but no 

 true galls. 



CHESTNUT ON SUGAR BEET. 



On November 7, 1908, among a lot of chestnut trees received by 

 the Department of Agriculture there was one with a gall 3 inches in 

 diameter on the stem. This gall was rather soft and of a texture 

 much like that of a nut meat. Plates were poured from it and tlie 

 gall colonies appeared 4 days aftervv'ards. 



Inoculations of November 13, 1908 (Brown). 



Five J^oung sugar beets were inoculated with colonies from the 

 })lates poured November 7, which colonies were 2 days old (visible 

 2 days). The beets were grown in an open bed in the greenhouse. 



Result. — December 26, 1908: All the inoculated sugar beets had 

 knots three-fourths to 1 inch in diameter (PI. II, fig, 4). They were 

 white and not so hard as the ordinary galls of daisy and peach. 



January, 1909: The galls on the sugar beets rotted off before the 

 end of the second month. 



Inoculations of May 24, 1910 (Brown). 



Five medium-sized sugar beets were inoculated from an agar 

 streak by needle pricks on the root. 

 Result. — July 18: All negative. 



Inoculations of June 10, 1910 (Brown). 



A fresh isolation called ''new chestnut" was made out of a rather 

 old gall procured from the estate of Mr. David Fairchild, in Maryland. 

 Five beets were inoculated on the roots by needle priclvs. 



Result.— Su\j 18, 1910: Negative. 



POPLAR ON OLEANDER. 



Inoculations of July 20, 1910 (Smith). 



Six young shoots on vigorous plants were inoculated by needle 

 pricks from a peptone water culture 5 da3"s old. 



Result. — October 22, 1910: One shoot misshig; the other 5 diseased, 

 but only in the pricked area. The results in detail are as follows: 

 (1) Four rounded knots (one-eighth to one-half inch in diameter) ; 16 



2 IP. 



