94 CROWN-GALL OF PLANTS. 



remain in the bed until this date that they miglit get larger. Each 

 of the 10 plants now bears a good-sized tumor, and at least half are 

 larger than the root which bears them (PL XXII, B, 0). The surface 

 is white or pinkish, and they have not yet begun to decay. Material 

 for sections was fixed in Carnoy. No checks were held, but the 

 adjoining row inoculated at about the same date with an isolation 

 from a chestnut gall might be considered as a check row, since the 

 numerous needle pricks have yielded notliing. 



Remarks. — Cultures were also made from the large old poplar gall 

 shown on Plate XXIII, and subcultures from 2 of the most hopeful 

 looking colonies (called Newport No. 1 and Newport No. 2) were 

 inoculated into sugar beets (upper part of root) by needle pricks on 

 June 30, 1910, but without results. Nine plants were inoculated, 

 but the weather was hot and the}^ did not grow much. The exami- 

 nations were made on July 18. 



POPLAR ON CALLA. 



Inoculations of July 5, 1910 (Brown). 



The corms of 8 growing callas were inoculated with 4-day-old agar 

 cultures of the Flats poplar organism. Both leaves and corms were 

 in good condition. 



Result. — October 25, 1910: Examined the callas and found a 

 pebble-like outgrov/th on 1 of those inoculated; also knobbed portions 

 on 2 others. Plates were poured from 2 corms, but no gall colonies 

 appeared. 



WILLOW ON DAISY. 



Inoculations op May 9, 1910 (Brown). 



In the spring of 1910 a small willow gall was received from South 

 Africa. Plates were made from it, and colonies obtained which 

 resembled Bactermm tumefaciens. A subculture from one of these 

 colonies was pricked into 3 terminal shoots of old, slow-growing 

 daisies already bearing large daisy galls near the ground. 



Result. — June 25, 1910: Two of the shoots bear smooth brown 

 galls one-half inch or more in diameter at the place inoculated. The 

 third shoot, which is yellow and sickly, has not developed any. 



WILLOW ON WILLOW. 



Inoculations of December 12, 1910 (Smith). 



Six recently rooted small cuttings of Salix hahylonica were inocu- 

 lated on rather slow-growing young shoots by needle pricks, using 

 a 4-day agar streak culture which was a subculture (possibly the 

 twelfth) from a colony i)lated from the South African willow gall 

 in December, 1909. 



213 



