EXPERIMENTS WITH THE APPLE OEGANISM. 99 



Inoculations of February 24, 1908 (Smith). 



Three Wealthy apple trees were moculated with 4-day-old slant 

 agar cultures (from beef-bouillon culture of Febi-uary 18, from stock 

 agar stab of January 6) of Hedgcock's first (D. C.) apple gall. Four 

 inoculations were made on each plant, 2 into young shoots and 2 into 

 old stems. 



Result. — June 1, 11)08: No tumors. Same set of j)lates as the 

 preceduig. 



Inoculations of October 22, 1908 (Brown). 



Three apple trees were inoculated on the crown and on the stem 

 from hard-gall colonies on plates poured October 15. The colonies, 

 however, had not appeared until October 20, so that for the most part 

 the cultures were in reality only 2 days old. The apple trees were 

 small and in a poor condition. 



Result. — November 24, 1908: Two of the 3 apple trees had small 

 galls at the crown. One of these trees bore 2 galls, one at the crown 

 and the other a little below the crown. • 



December 22, 1908 (see PI. II, fig. 2). 



Inoculations of November 12, 1908 (Smith). 



Four apple trees were inoculated from colonies on poured plates 

 made November 4 from Doctor Hedgcock's first Iowa apple gall. When 

 used for inoculation the colonies were white, dense, fleshy, circular, 

 wet-shining. The trees were leafy but growing very slowly. They 

 aie of the same lot that failed to take daisy inoculation (p. 43), and 

 earlier root inoculations (January 23, 1908) with organism from 

 fust (D. C.) hard gall of apple. Each of the 4 trees was inoculated 

 on the root just underground; 2 of them on 2 roots each, and 2 also 

 on parts aboveground — 1 in 1 place and the other in 3 places. 



Result. — Nothing. Trees growing very slowly. The same organ- 

 ism inoculated into daisies gave slow-growing hard galls. 



HARD GALL OF APPLE ON SUGAR BEET. 

 Inoculations of November 12, 1908 (Smith). 



Nine sugar beets were inoculated from poured-plate colonies (each 

 from a separate colony) made November 4 from first hard gall of apple 

 received from Iowa. At the time of inoculation the colonies were 

 white, dense, fleshy, circular, wet-shining. 



Result. — Negative. Plants small and making scarcely any growth, 



213 



