104 CROWN-GALL OP PLANTB. 



Result. — December 2, 1908: Examined the beets and found that 

 fme roots were growing out at the punctured places, and little warty 

 growths were at the bases of these roots. One and sometimes 2 

 roots protruded from a punctured place. Tliis was observed on 

 4 out of the 6 beets. 



December 22, 1908: Hairy roots were found on 1 more of the 

 inoculated beets, making 5 out of the 6. No galls like the distinct 

 galls of the daisy, peach, or apple were produced. 



The daisy did not develop either hairy root or galls, although it 

 was under observation until April 17, 1909. 



Inoculations of December 22, 1908 (Brown). 



Eleven young sugar beets were inoculated with slant agar cultures 

 of the hairy-root organism 3 days old, the second subculture from 

 agar poured-plate colonies. The organism was obtained from the 

 apple tree brought in by Doctor Hedgcock. The inoculations were 

 made just below the surface of the soil. 



Result. — January 9, 1909: Four of the beets were pulled up; 

 clustered roots of rather a fleshy texture were found on all 4 at the 

 place of inoculation. There was no possibility of confusing these 

 roots vv'itli those that occur regularly on either side of the beet, for 

 they were too near the crown of the beet and besides were growing 

 from small nodules. 



April 10, 1909: Three more beets were removed and examined. 

 Each one bore typical hairy-root (clustered roots) at the point of 

 inoculation, which was midway between the two lines of lateral 

 roots (PL XVII, flgs. 1 and 2). ' 



April 29, 1909: The remaining four beets were removed and 

 examined. Three of these showed undoubtetl hairy-root at the point 

 of inoculation. The fourth one probably developed hairj^-root, but 

 was rejected from the count because the needle entered on the line 

 of the lateral roots rather than on the smooth surface between them, 

 the inoculation having been made when the plant was very small. 



Inoculations of February 24, 1909 (Brown). 



Seven small sugar beets were inoculated just below the surface of 

 the ground with 1-day-old agar streak cultures of the apple liairy- 

 i-oot organism, the first subculture from poured-plate coloni(^s ob- 

 tained from one of the apple trees sent from New York by Doctor 

 Whetzel. 



Result. — Marcli 10, 1909: Two beets were pulled up and examined; 

 clustered roots were found on one of them at the inoculated places, 

 which were on the smooth part of the beet. 



213 



