MORPHOLOGY OF THE DAISY ORGANISM. 



107 



Fig. 1. — Flagella of Bacterium tume- 

 faciens from daisy: a, Pitfield's 

 flagella stain; h, Van Erraengem's 

 stain; c, Loeffler's flagella stain; d, 

 from a slide stained 30 minutes in 

 carbol fuchsin without mordant. 



stained by Miss Brown, using Loeffler's flagella stain (fig. 1, c), and 

 subsequently Van ll^rmengem's stain (fig. 1 , i). They were also stained 

 by Miss Lucia McCulloch without a special mordant, by simply 

 exposing the flamed covers to carbol fuch- 

 sin for from 30 to 60 minutes and then 

 washing in alcohol (fig. \,d). 



Capsules (?). — The organism is viscid 

 after some days on agar, etc., but capsules 

 have not been demonstrated. Welch's 

 stain w^as tried. 



Zooglccse ( ?). — Pseudozoogloeffi occur, and 

 perhaps the stringy masses in peptonized 

 beef bouillon should be regarded as transi- 

 tions toward zoogloese. Under the micro- 

 scope these masses consist of short rods 

 held together bv a viscid slime. 



Involution forms. — Numerous involution 

 forms (fig. 2) were observed in bouillon 

 cultures which were making a slow growth 

 at 0° C. The cultures were first examined under the microscope 

 on the fourteenth day. Occasional Y-shaped rods occur in young agar 

 cultures (fig. 1, a). Club-shaped and Y-shaped involution forms were 

 also seen in salt bouillon and in bouillon and agar to which acetic 



acid was added. See also note on ordinary 

 bouillon. 



BEH.A.VICII TOWARD STAINS. 



This organism when taken from young 



agar cultures stains readily in all ordinary 



/^ c^^^^^^^=:^ y-O "^^^^ basic anilin stains so far as tried, e. g., 



"^^"--^ '^ gentian violet, fuchsin, carbol fuchsin, 



amyl Gram, methyl violet. It is not sur- 

 rounded by any substance that interferes 

 with staining. When stained from a 2-day 

 agar streak in Loeffler's alkaline methy- 

 lene blue, the rods were either a uniform 

 pale blue or showed round to oval, inner 

 portions bearing a much heavier stain. 

 There were sometimes two of these bodies 

 in a rod, but more often one and that 

 usually polar. About one-fourth of the rods stained in this manner 

 and the part not heavily stained was of a uniform pale blue. 



213 



Fig. 2. — InvolLitiou forms of daisy 

 organism after two weeks in bouil- 

 lon at 0° C. Bottom growth: a, 

 drawn by E. F. S.; b, drawn by 

 Brown. Similar involutions forms 

 were produced in young agar cul- 

 tures and also in bouillon by ex- 

 posure to acetic acid. 



