CULTURAL CHAEACTERS OF THE DAISY ORGANISM, 113 



did not take stain very readily (carbol fuchsin). No tyrosin crystals 

 were found (hand lens and compound microscope), and if there is any 

 solution of the curd it is very slow and incomplete. 



At the end of 10 days, tubes of milk inoculated with the daisy organ- 

 ism in February, 1910, showed about 2 mm. depth of whey on top of 

 a separating curd, which remained fluid. At the end of the twenty- 

 fourth da}^ there was a small amount of clear whey over a copious 

 fluid curd, beneath which was a small amount of clear white bacterial 

 precipitate. Color much as in checks, w^hich w^ere brownish from 

 overheating. 



On j\Ia3' 9, 1910, 6 additional inoculations were made from 3 of the 

 6-months-old milk cultures into sterile white milk (i. e., milk not over- 

 heated), 3 check tubes being held. At the end of 9 days the only 

 visible change was a white pellicle on the inoculated milks. Exam- 

 ined July 1, the inoculated tubes contained about 1 centimeter depth 

 of clear whey supporting a well-defined white pellicle and resting on 

 a homogeneous-looking opaque white curd about 3 centimeters deep 

 fPl. XXV, figs, g, h). The curd was not browned and yet not as 

 white as in the checks. This so-called curd was fluid, as shown by 

 gentle shaking. 



There is never any rapid separation and digestion of the curd such 

 as Brizi describes for his Bacillus populi. 



LITMUS MILK. 



The litmus is gradually hlued, then reduced. Inoculations into litmus 

 milk, using a 1-mm. loop of a 3-day-old beef-broth culture, resulted 

 in 8 days in a deeper blue color (indigo blue) ; and in 24 days the blue 

 color disapjoeared with a slight formation of whey at the surface. 

 It is apparent from this test and others which were subsequently 

 instituted (Table XII) that the culture is alkaline from the start, the 

 litmus becoming reduced later. The litmus is never reddened. The 

 behavior in litmus milk indicates the presence of a lab ferment. The 

 reduction of the litmus may be partial or complete and is always slow. 

 There was not much, if any, peptonization of the curd, and the whey 

 at the end of 2 months was dark by reflected light (not red), the curd 

 being either bluish, drab, or wholly bleached. 



SILICATE JELLY. 



Slow white growth. (See p. 156.) 



cohn's solution. 



GroivtTi scanty or absent, medium nonfiuorescent. Many tests were 

 made. 



78026°— Bull. 213—11 8 



