CULTURAL CHARACTERS OF THE DAISY ORGANISM. 115 



indicates ability of the organism to take botli its nitrogen and its 

 carbon from asparagin. A decidedly less amount, but some growth, 

 was obtained in river water containing only dextrose. In river water 

 alone no growth was obtained. For further data see tables under 

 Observed Differences in Organisms from Various Sources. 



BEST MEDIA FOR LONG-CONTINUED GROWTH. 



Milk, bouillon, dextrose peptone water with calcium carbonate are 

 the best media we have tried. In tubes of milk the organism has 

 lived for six months. 



QUICK TESTS FOR DIFFERENTIAL PURPOSES. 



The following are recommended tests: 



Gelatin and agar plates, especially time of appearance of colonies on 

 4-15 agar plates made. from the tumors; young agar stroke cultures; 

 behavior in milk and litmus milk; growth on potato; behavior in 

 Cohn's solution; behavior in the thermostat at 37° C; stringy ring 

 and suspended filaments in peptonized beef bouillon; inoculations 

 into young, rapidly growing daisy shoots or into growing sugar-beet 

 roots. 



FERMENTATION TUBES. 



No gas is produced, and the organism is aerobic in its tendencies. A 

 basal solution was made by adding 2 per cent of Witte's peptone to 

 water. Six solutions were then made from this, each containing 

 1 per cent of the following carbon compound : Glycerin, cane sugar, 

 mannit, dextrose, maltose, lactose. One-half dozen fermentation 

 tubes were filled with each of these solutions and sterilized by heating 

 20 minutes on three days in succession. Four tubes of each set were 

 inoculated and 2 were left for control. The inoculated tubes each 

 received a 1 mm. loop from a 2-day-old culture growing in water 

 containing 2 per cent Witte's peptone and 1 per cent glycerin. Four 

 days after inoculation there was a slight cloudiness in the open end 

 of all inoculated tubes. The clouding was most conspicuous in the 

 tubes containing dextrose and this extended down to the elbow. 

 Next to the dextrose in point of cloudiness stood the maltose with 

 threadUke thickenings floating at the surface. At the end of 10 

 days the cloudiness in the dextrose tubes had extended slightly into 

 the closed end. A distinct deposit had also formed and particles of 

 soUd matter were floating in the hquid in the clouded part of the 

 tube. Maltose had clouded to the middle of the U; the mannit 

 solutions were clouded shghtly beyond the U into the closed end of 

 the tube, and a distinct deposit had formed. The cane-sugar and 

 milk-sugar solutions were clouded almost to the bend in the tube, and 



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