128 CKOWN-GALL OF PLANTS. 



heated for 1 hour at 80° to 86° C, after which there was no growth 

 (tubes under observation 23 days). 



The name denotes the kind of gall from which the organism was 

 isolated. 



NEWEST DAISY. 



A culture recently isolated and still infectious. 



(a) Size 0.6 to 0.8 by 1.2 to 2.0/i. Most, I think, about 1.5/x long. 

 Many short chains (4 to 8 segments) occur. Staining irregular and 

 the protoplasm so pulled apart that it is very difficult to decide on the 

 extreme length. Occasional club-shaped rods occur. 



(b) Repetition two days later (unflamed): Size 0.5 to 0.7 by 1.0 to 

 3.0//. Stained uniforml}^ The rods are single, paired, or in short 

 chains often with indistinct constrictions, making it difficult to deter- 

 mine the maximum length of rods. The longest with indistinct 

 septation are 4 to 10/x. A few are twice as broad as the multitude; 

 a few are branched; a few are club shaped. 



Acid fast. — Negative. Agar 6 days. 

 Gram's slain. — Negative. Agar sti'eak 4 days. 

 Flagella. — Polar, 1 to 3. Pitfield's stain. 

 Spores. — Negative. 



OLD DAISY (b). 



A strain not now infectious: Size 0.4 to 0.6 by 1.0 to 2A/n. Looks 

 like the right thing. The bacteria adhere on the cover in small 

 clumps. They are often paired and occasionally 4 are joined end to 

 end. There is also an occasional club-shaped rod. 



Add fast. — Negative. Agar 5 days. 

 Gram's stain. — Negative. Agar 5 days. 

 Flagella. — Polar, 1 to 3. Pitfield's stain, etc. 

 Spores. — Negative. 



PEACH. 



Size 0.5 by 1.0 to 2.0/<. 



Acid fast. — Negative. Agar 5 days. 

 Gram's stain. — Negative. Agar 5 days. 

 Flagella. — Polar, 1 to 3. Pitfield's stain. 

 Spores. — Negative. 



HOP. 



Size 0.4 to 0.8 by 1.0 to 1.6/(. Occasional club-shaped rods are 

 present, but they are much less numerous than in the grape. 



Add fast. — Negative. Agar 6 days. 

 Gram's stain. — Negative. Agar 5 days. 

 Flagella. — Polar, 1 to 3. Pitfield's stain. 

 (Spores.— Negative. 

 213 



