MORPHOLOGY OF GALL ORGANISMS FROM VARIOUS SOURCES. 129 



NEW ROSE. 



Size 0.5 to 0.6 by 1.0 to 2.5/^, rarely 3//. 



Acid fast. — Negative. Agar 5 days. 

 Gram's stain. — Negative. Agar 5 days. 

 Flagella. — Polar, 1 to 2. Pitfield's stain. 

 Spores. — Negative. 



OLD ROSE. 



No measurements. 



Acid fast. — Negative. Agar 5 days. 

 Gram's stain. — Negative. Agar 5 days. 

 Flagella. — Polar, 1 to 3. Pitfield's stain. 

 iSpores. — Negative. 



OLD APPLE. 



Size 0.6 to 0.8 by 0.6 to 0.8/i. Often 3 to 8 elements in a chain. 

 Almost like a streptococcus. Does not look like the right thing. 

 Probably an intruder which has displaced the original pathogenic 

 organism. Recent inoculation tests on sugar beet (June, 1910) gave 

 negative results. (See also Cultural Characters.) 



Acid fast. — Negative. Agar 5 days. 

 Gram's stain. — Positive. Agar 5 days. 

 Flagella. — Unable to stain. 

 Sp ores. — Negative . 



APPLE HAIRY-ROOT. 



Size 0.4 to 0.7 by 1.0 to 2.0/<. Occasionally one thicker. 



Acid fast. — Negative. Agar 7 days. 

 Gram's stain. — Negative. Agar 5 days. 



Flagella.— Volar, mostly 1 flagellum (Loeffler's stain). No results with Pit- 

 field's stain. 

 Spores. — Negative. 



NEW APPLE. 



Descended from apple hairy-root, i. e., from apple gall which bore 



no roots but which produced both galls and hairy roots on sugar 



beet: Size 0.4 to 0.8 by 1.0 to l.Qpt. Occasionally one may be longer. 



Acid fast. — Negative. Agar 6 days. 

 Gram's stain. — Negative. Agar 4 days. 

 Flagella. — Unable to stain. 

 Spores. — Negative. 



ALFALFA. 



Size 0.4 to 0.7 by 0.8 to 2.0/i. Average length about 1.2//. Most 

 are 0.5 to 0.6// in diameter. In those which are 1.8 or 0.2// long a 

 slight equatorial constriction is usually visible. Occasional club- 

 shaped rods are present. 



Acid fast. — Negative. Agar 6 days. 

 Gram's stain. — Negative. Agar 5 days. 

 Flagella.— Tolai, 1 to 2. Pitfield's stain. 

 Spores. — Negative. 



78026»— Bull. 213—11 9 



