130 CROWN-GALL OF PLANTS. 



GRAPE. 



Size 0.5 to 0.9 by 1.0 to 1.5/<. Swollen and club-shaped rods 1.2 

 to 1.3/j in diameter are frequent. Perhaps degeneration forms." 



Acid fast. — Negative. Agar 5 days. 



Gram's stain. — Negative. Agar 4 days. 



Flagclla. — Polar, 1 to 2. Van Ermengem's stain. No result with Pitfield's 



stain or Loeffler's stain. 

 Spores. — Negative. 



NEW CHESTNUT. 



Size 0.5 to 0.7 by 0.8 to lAfi. Seems to be rather shorter than 

 most. The average length is about 1.0 to 1.2/^ long. An exception- 

 ally long pair measures 2.8//, another average pair 2.2//. The rods 

 are single, in pairs, 4's or 8's, with distinct rounded constrictions. 

 Pathogenicity not proved. Beets and grape inoculated gave no con- 

 clusive results. 



Acid fast. — Negative. Agar 6 days. 

 Gram's stain. — Negative. Agar 5 days. 

 Flagella. — Unable to stain. 

 Spores . — N egati ve . 



ARBUTUS UNEDO. 



Size 0.5 to 0.9 by 1.2 to 2.2//. 



Acid fast. — Negative. Agar 5 days and bouillon 14 days. 

 Gram's stain. — Negative. Agar 5 days. 

 Flagella. — Unable to stain. 

 Spores. — Negative. 



COTTON. 



Size 0.4 to 0.6 by 1.0 to 2Afi. Rarely as long as 2.4/« and most 



about 0.5/( in diameter. A few are club-shaped; a few are broader, 



and deeper stained than the majority. Slide overwashed. 



Acid fast. — Negative. Agar 6 days. 



Gram's stain. — Very feeble stain — should be estimated as negativeis Agar 4 



days. 

 Flagella. — Polar, 1 to o. Pitfield's stain. 

 /Spores . —N egati ve . 



QUINCE. 



Size 0.5 to U.7 by 1.2 to 2.()/(. Greater tendency to short chains 



than in most of the slides so far examined, i. e., like newest daisy, but 



constrictions plainer. 



.4cirf/asL— Negative. Agar 7 days. 



Gram's stain. — Negative, i. e., very feebly stained. Agar 1 day. Positive 



(same strain), agar 5 days. No satisfactory inoculations. 

 Flagella. — Polar, mostly 3. Stained by Loeffler's stain. No results with 



Pitfield's stain. 

 Spores. — Negative. 



a After 2 weeks on agar slant, Y's and swollen rods were very common, i. e., much more so than In 

 any of the other 24 examined. 

 213 



