MORPHOLOGY OF GALL ORGANISMS FROM VARIOUS SOURCES. 131 



SUGAR BEET. 



Organism which shows slightly jiinkish on agar after a few days 

 and is not infectious: Size 0.5 to 0.9 by 1.2 to 2.5//. Two well- 

 developed rods not 3"et separated measure together 0.8 by 3.2/x. 

 Rods 2.5/f long without any distinct constriction are frequent. 



Acid fast. — Negative. Agar 6 days. 

 Gram's stem. —Negative (feeble Btain). 

 Flagella. — Unable to stain. 

 Spores. — Negative. 



WILLOW FROM SOUTH AFRICA. 



Size 0.4 to 0.7 by 1.2 to 2.4/i. Most of the rods are 1.5 to 2.0/i long. 



They are single or in pairs, occasionally in 4's joined end to end. The 



pairs frequently are curved a little. There are occasional club-shaped 



rods. 



Acid fast. — Negative. Agar 5 days. 

 Gram's stain. — Negative. Agar 3 days. 

 Flagella. — Unable to stain. 

 Spores. — Negative. 



POPLAR (flats). 



Size 0.3 to 0.6 by 1.0 to 1.8//. Most are 0.4 to 0.5/« in diameter. 

 No spores are present. 



Add fast. — Negative. Agar 6 days. 

 Gram's stain. — Negative (a feeble stain). 

 Flagella. — Polar, 1 to 2. Pitfield's stain. 

 Spores. — N egative . 



POPLAR (NEWPORT, R. I.). 



Two colonies, which looked alike, were transferred from the ])oured 

 plate, but they are not alike in morphology. 



Colony 1. — Size 0.7 to 1.0 by 1.0 to 1.2/<. A very short, plump rod, 

 with rounded ends, almost a coccus form, mostly paired. Not yet 

 proved up and doubtful if the right organism. It has been inoculated 

 into sugar beet with negative results. 



Acid fast. — Negati\e. Agai* 5 days. 

 Gram's stain. — Negative. Agar 5 days. 

 Flagella. — Unable to stain. 

 Spores. — Negative. 



Colony ^.— Size 0.4 to 0.5 by 0.8 to 2.0 ii. Mostly 1.0 to 1.4/i long. 

 The rods are longer than those of colony 1. Sugar beet inoculated 

 unsuccessfull}^ ; pathogenicity not yet determined. 



Acid fast. — Negative. Agar 4 days. 

 Gram's stain. — Negative. Agar 3 days. 

 Flagella. — Polar, 1 to 2. Pitfield's stain. 

 Spores . — Negative . 



213 



