CHARACTERS OF GALL ORGANISMS FROM OTHER SOURCES. 141 



(4) A thill white growth iiichned to wriiikk^ crystals few and large: 

 Quince. This is the only culture showing any wrinkles. 



This experiment was repeated in July, 1910, at a higher temperature 

 (30° to 31° C), inoculating from younger cultures (1 to 3 day agar), 

 with less typical growth (in some cases colony wise) but nevertheless 

 with essentially the same results. Under group 2 fewer with crystals. 

 The old apple looks decidedly unlike the others and is probably an 

 intruder. 



GROWTH IN +16 BEEF BOUILLON WITH 1 PER CENT WITTE'S 



PEPTONE. 



At the end of 3 days at 23° to 25° C, inoculating from slant agar 

 cultures 18 days old, the appearances in test tubes containing 10 c. c. 

 of the fluid were as follows: 



(1) Incomplete easily fragmenting pellicle, fluid nearly clear, 

 stringy threads on shaking: Arbutus, alfalfa, newest daisy. 



(2) Cloudier and with a heavier pellicle but otherwise like 1 : New 

 rose, old rose, cotton, hop, old daisy (B), peach. 



(3) Cloudy and more or less stringy but destitute of pellicle, some 

 precipitate: New apple, old apple, apple hairy-root, beet. 



(4) Cloudy with some flocks, but no pellicle or strings: Quince." 

 This experiment was repeated in July, 1910, at 30° to 31° C, 



inoculating from 3-day-old bouillon cultures. The results at the end 

 of 3 days were the same, except that now the newest daisy had no 

 pellicle, and the old rose an easily fragmenting pellicle. The previ- 

 ously untried strains fell into the above-named groups as follows: 



Group 1. — Newport poplar No. 1, salsify. 

 Group 2. — Turnip No. 1. 

 Group S. — Grape, Newport poplar No. 2. 

 Group 4- — New chestnut, willow. 



The parsnip did not resemble the others. It was heavily and uni- 

 formly clouded with a precipitate which rose in a swirl on shaking. 

 There were no strings, rim, or pellicle. 



CANE-SUGAR PEPTONE WATER. 



When grown for 1 8 days in river water containing 2 per cent Witte's 

 peptone and 2 per cent c. p. cane sugar the strains did not brown the 

 fluid, but behaved as follows: 



(1) Fluid clear and not much precipitate or flocculence, but a 

 very copious thick white pellicle (0.5 to 1.5 cm. thick, mostly the 

 latter): Alfalfa, turnip No. 1, Flats poplar, new rose (pellicle 0.5 cm.) 

 cotton, hop, peach, old daisy, newest daisy. 



a The old chestnut was discarded as contaminated, and the grape did not grow. 

 213 



