CHARACTERS UF GALL ORGANISMS i'ROM OTllLK SOURCES. 



143 



(4) Fluid dried out one-third. Dense white slimy pellicle nearly 

 filling the remainder of the fluid, i. e., 2 cm. deep, not much precipi- 

 tate, fluid alkaline: Old daisy, hop. 



(5) Exactly like the preceding except that fluid and pellicle are 

 slightly brownish, fluid alkaline: Peach, cotton. 



(6) Thinly clouded, no rim, or pellicle, fluid unstained, a moderate 

 precipitate which shakes up in a coarse flocculence, which is most 

 abundant in the beet: Old apple (acid to neutral litmus paper), beet 

 (strongly alkaline), old chestnut (neutral to litmus). 



(7) Fluid moderately cloudy and yellowish (alkaline to litmus), 

 rim yellowish wliite, not stringy, no pellicle, moderate precipitate 

 which shakes up in coarse flocculence: Quince. 



Table IV. — Showing behavior"' of crown-gall organisms in peptonized beef bouillon of 



varying grades of alkalinity or acidity. 



[Ihooulaled from 3-day-old and 7-day-old peptonized bouillon cultures, except —25, which was from 

 19-day-old beef bouillon. Examined at end of 28 or 31 days, exc«pt —25, which was 9 days old. The +34 

 was acidulated with citric acid, the +38 with malic; the alkali was sodium hydroxide.] 



a Explanation of figures indicating growth: 0=no growth; l=trace; 2=slight; 3=moderate; 4=good; 

 5= copious. 

 6 Thick firm pellicle, not broken by shaking. 



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