144 



CEOWN-GALL OF PLANTS. 



Some additional tests were made in 1910 in acid and alkaline pep- 

 tonized beef bouillon with the results shown in Table V. 



Table V. — Showing behavior" of croiun-gall organisms in bouillons of varying reaction, 

 the records being taken some xveeks after inoculation. 



[The alkali was sodium hydrate.] 



a Explanation of figures indicating growth: 0= no growth; 2= slight; 4= good, with pellicle. 



Table VI. — Showing behavior o of crown-gall organisms in -\-15 bouillon containing 



sodium chloride at room temperatures. 



(Transfers from 5-day-old bouillon cultures.] 



Organism. 



Daisy: b 



3.0 per cent 



3.5 per cent 



4.0 per cent 



Peach: 



3.0 per cent 



3.5 per cent 



4.0 per cent 



Hop: 



3.0 percent 



3.5 per cent 



4.0 per cent 



Rose: 



3.0 per cent 



3.5 per cent 



4.0 per cent 



Apple: 



Old strain - 

 3.0 percent. 

 3.5 per cent . 

 4.0 percent. 



New strain— 

 3.0 per cent. 

 3.5 percent. 

 4.0 per cent. 

 Apple hairy-root: 



3.0 per cent 



3.5 per cent 



4.0 per cent 



Alfalfa: 



3.0 percent 



3.5 per cent 



4.0 per cent 



Time (days). 



4. 15. 31 



Organism. 



Grape: 



3.0 per cent. 



3.5 per cent. 



4.0 per cent. 

 New chesnut: 



3.0 per cent. 



3.5 percent. 



4.0 percent. 

 Old chestnut: 



3.0 percent. 



3.5 per cent. 



4.0 per cent. 

 Arbutus: 



3.0 per cent. 



3.5 per cent. 



4.0 percent. 

 Cotton: c 



3.0 per cent . 



3.5 per cent . 



4.0 per cent. 

 Beet: 



3.0 percent. 



3.5 per cent. 



4.0 per cent. 

 Quince: 



3.0 per cent. 



3.5 per cent. 



4.0 percent. 

 Flats, poplar: 



3.0 percent. 



3.5 per cent. 



4.0 per cent. 



Time (days). 



15. 31 



2 

 

 





 

 



5 

 1 

 



5 

 4 

 4 





 

 



a Explanation of figures indicating growth: 0=no growth; l=very slight; 2=sllght; 3= fairly good. 

 4=good; 5= heavy. 



b In a repetition of daisy there wa,s moderate growth at end of six weeks In 3 and 3.5 per cent. 



cln a repetition of cotton there was no growth at end of three days and at end of fifteen days; at end 

 of forty days the growth was 3, 3, and 0, respectively, for 3, 3.5, and 4 per cent. 



213 



