CHABACTERS OF GALT. ORGANISMS FROM OTHER SOURCES. 149 



end set of plates poured and jiortions of 11 typical-looking colonies 

 transferred to as many tubes of the bouillon. Tubes tested on third 

 day, when well clouded, all negative. These tubes foamed on 

 shaking; the check tube did not. Before the reagents were added, 

 the fluid in the check tube was neutral (or nearly so) to litmus, and 

 that in the inoculated tubes strongly alkaline. 



HOP. 



Old tests hy Miss Brown. — (1) 13 days, negative; (2) 5 days, re- 

 duced; (.3) 33 days, trace of blue which disappeared on shaking. 



Tests in 1910 hy Doctor SmitJi. — (4) 69-day-old culture by Miss 

 Brown in her nitrate bouillon No. 600 (fluid strongly alkaline to lit- 

 mus). On adding the reagents, a trace of blue in bottom, which 

 shakes out.'^ 



ROSE. 



Old tests hy 2Iiss Broivn. — (1) 13 days, reduced; (2) 5 days, reduced 

 (both new and old strains); (3) 33 days, reduced (both strains). 



Tests in 1910 hy Doctor Smith. — (4) GO-daj^-old culture by Miss 

 Brown in her nitrate bouillon No. 600: New rose — Copious reduction; 

 does not shake out. Old rose (strongly alkaline to litmus) — Copious 

 blue reaction, does not shake out; reagents tested and found pure. 

 (5) New rose — Plates poured and portions of 10 colonies transferred 

 to as many tubes, also 3 tubes inoculated from mixed colonies; tubes 

 tested when 5 days old and fluid well clouded; one check tube, nega- 

 tive; of 10 tubes, 7 gas forming, 3 non-gas forming; the latter did not 

 reduce; the former showed trace of blue on pellicle where acid fell; 

 of the 3 tubes from mixed colonies, 1 reduced, 2 did not.'' (6) Old 

 rose — Plates poured and portions of 8 colonies transferred to as many 

 tubes of bouillon; two checks held; tests after a week; fluid heavily 

 clouded and contamination suspected. Result — Checks negative; of 

 the inoculated tubes, 4 show a trace of blue on the bottom, which dis- 

 appears on shaking, 2 are heavily blued throughout, and 2 are inter- 



a A fresh isolation from hop made in 1910, and pathogenic to daisy and sugar beet, was tested as follows 

 for nitrate reduction: 



Five tubes at end of 7 days' growth (colony 1), negative. Two tubes of same lot at end of 27 days gave 

 a very strong reduction. Contamination was then suspected and poured plates were made. At the end 

 of .3 days 12 colonies were transferred to nitrate bouillon. These cultures were tested at the end of 10 days, 

 when the bouillon was well clouded. Ten gave no reduction, 1 gave a slight color which shook out, 1 

 reduced moderately, color persisting. Transfers from these 12 tubes were made to plain bouillon before 

 testing, and from these bouillon cultures other 12 tubes of nitrate bouillon were inoculated on January 

 27 and tested at end of 14 days, when all were moderately clouded and bore a heavy pellicle which frag- 

 mented easily on shaking. The result on adding 10 drops of boiled starch water, 1 c. c. of 1 : 250 fresh potas- 

 sium iodide water, and 5 drops of 2 : 1 sulphuric acid water were as follows: Two tubes, no reduction; 10 

 tubes, blue reaction in precipitate (fallen pellicle), in two of these there was also a trace of blue at the top 

 of the fluid. The mass of the fluid was entirely free from blue color, and all of the color disappeared on 

 shaking. 



6 Growth on agar slant August 3, 1910, looked wrong, i. e., it was pinkish white. Examined after 19 

 days. 



213 



