150 CROWN-GALL OF PLANTS. 



mediate, i. e., one blue at bottom, shaking out, the other remaining 

 pale blue after shaking. (7) Old rose — Second set of plates poured 

 and portions of 11 colonies transferred to as many tubes; cultures 

 tested on tliird day, when well clouded. Result — Check negative ; 11 

 inoculated tubes all blue at bottom in bacterial precipitate; 1 tube 

 remained pale blue after shaking; the other 10 became colorless; on 

 shaking, the inoculated tubes became half filled with foam. 

 Conclusion: New rose, contaminated; old rose, doubtful. 



APPLE. 



Old tests hy Miss Brown. — (1) 13 days, reduced; (2) 5 days, old 

 apple, reduced; new apple, negative, possibly no growth; (3) 33 

 days, old apple, reduced; new apple, trace (?), gave scarcely any 

 growth. 



Tests in 1910 hy Doctor Smitli. — (4) 69-day-old culture by Miss 

 Brown in her bouillon No. 600 (fluid strongly alkaline), copious blue 

 reaction which does not shake out; the new apple gave no growth. 



APPLE HAIRY-ROOT. 



Old tests hy Miss Brown. — (1) 13 days, negative; (2) 5 days, re- 

 duced; (3) 33 days, trace(?), scarcely any growth. 



Tests in 1910 hy Doctor Smith. — (4) Plates poured and portions of 

 12 colonies transferred to as many tubes of the bouillon. The tests 

 were made at the end of 13 days. One tube is cloudy with a moder- 

 ate rim and precipitate, but the others show scarcely any growth. 

 Result: All negative; no reduction. 



ALFALFA. 



Old tests hy Miss Brown. — (1) 13 days, reduced; (2) 5 days, re- 

 duced; (3) 33 days, trace of blue which disappears on shaking. 



Tests in 1910 hy Doctor Smith. — (4) 69-day-old culture by Miss 

 Brown in her bouillon No. 600 — blue in bottom on adding the rea- 

 gents (color shakes out). (5) Plates poured and portions of 12 

 colonies transferred to as many tubes of the bouillon; cultures tested 

 when 5 days old and well clouded; check, negative; 12 cultures, all 

 negative. 



GRAPE. 



Old tests hy Miss Brown. — (1) 13 days, reduced; (2) 5 days, nega- 

 tive; (3) 33 days, negative. 



Tests hy Doctor Smith in 1910. — (4) 69-day-old culture by Miss 

 Brown in her nitrate bouillon No. 600, moderate growth, negative. 



213 



