CHAKACTER OF GALL OIJGANISMS FROM OTHER SOURCES. 151 



OLD CHESTNUT. 



Old tests hy Miss Broion. — (1) 13 days, reduced; (2) .5 days, nega- 

 tive; (3) 33 days, negative. 



Tests in 1910 hy Doctor Smith. — (4) 69-day-old culture by Miss 

 Brown in her bouillon No. 600, negative; growth abundant. 



ARBUTUS. 



Old tests hy Hiss Brown.— (1) 5 days, reduced; (2) 33 days, trace 

 of color, scarcely any growth. 



Tests hy Doctor Smith in 1910. — (3) Plates poured and portions of 

 9 colonies transferred to as many tubes of the nitrate bouillon; 

 tested on twelfth day, when all had grown; fluid more or less stringy 

 and not much clouded, rim and pellicle fallen; all negative. The 

 presence of nitrate in each tube was then determined by obtaining 

 the evanescent blue reaction with diphenylamine dissolved in sul- 

 phuric acid. 



BEET. 



Old tests hy Miss Brown.— (1) 5 days, negative; (2) 33 days, re- 

 duced. 



Tests in 1910 hy Doctor Smitli. — (3) G9-day-old culture by Miss 

 Brown in her nitrate bouillon No. 600 (fluid strongly alkaline to lit- 

 mus) ; on adding the nitrite reagents a copious blue, which does not 

 shake out. (4) Plates poured and portions of 12 colonics transferred 

 to the bouillon; tubes tested on the thirteenth day, when there were 

 no strings or pellicle, but a white rim and a moderate amount of 

 pinkish white precipitate; check and 6 inoculated tubes, negative; 

 6 tubes blue at bottom over the thick bacterial precipitate, but color 

 shaking out of all. 



COTTON. 



Old tests hy Miss Brown. — (1) 5 days, reduced; (2) 33 days, reduced. 



Tests in 1910 hy Doctor Smith.— (3) 69-day-old culture by Mss 

 Brown in her nitrate bouillon No. 600 (fluid strongly alkaline) — 

 copious blue color which does not shake out. (4) Plates poured and 

 portions of 12 colonies transferred to as many tubes of nitrate bouillon. 

 The tests w^ere made at the end of 5 days when a good growth had 

 taken place. Eleven tubes, negative. One shows a blue color at the 

 bottom, but this shakes out. Presence of nitrate in these tubes was 

 then determined by the diphenylamine sulphuric-acid test. 



QUINCE. 



y 



Old tests hyMiss Brown.— (1) 13 days, negative; (2) 5 days, reduced; 

 (3) 33 days, trace of reduction, scarcely any growth. 



213 



