4i8 Bulletin 315 



The cultures were allowed to stand ten days. Transfers were then 

 made (exact method not stated) to culture solutions of the same composi- 

 tion as the original. These were again allowed to stand ten days and 

 another transfer made. Finally, a third transfer was made. Ten days 

 after, this third subculture was used for pouring dilution plates after the 

 Koch method. The media used for pouring plates were nonnal gelatin 

 (10 per cent gelatin, 10 per cent peptone, 0.2 per cent monopotassium 

 phosphate [KH2PO4], 0.2 per cent magnesium sulfate [MgSOj, 0.2 per 

 cent glucose), agar (1.5 per cent agar, i per cent peptone, 0.2 per cent 

 monopotassium phosphate [KH2PO4], 0.2 per cent magnesium sulfate 

 [MgSOj, 0.2 per cent glucose), and sodium humate mixed with gelatin. These 

 pure cultures now obtained were used in Nikitinsky's biochemical studies. 

 In a few instances cultures were obtained by inoculation of gelatin (without 

 humic acid) directly with soil. Further details of this method are not given. 

 The first method yielded mostly bacteria; one yeast is recorded. The 

 second method yielded Penicillium glaucum, aMucor, and a Trichothecium. 



Coming now to van Iterson ('04), we find the following method of pro- 

 cedure: Two sterile pieces of Swedish filter paper were placed in glass 

 dishes and these were moistened with tap water (leitungswasser) 100 cc, 

 ammonium nitrate 0.05 gram, monopotassium phosphate 0.05 gram. As 

 inoculation material he used soil or humus, but the best results were obtained 

 when the dishes were left exposed to the air for twelve hours. Following 

 inoculation of the plates they were maintained at 24° C. and the paper was 

 kept moist. He states that after fourteen days to three weeks a rich fungous 

 growth could be noticed. He now obtained pure cultures from these 

 plates for his further study. The exact methods pursued are not given. 



The following species were isolated: Sordaria humicola Oud., Pyronema 

 confluens TuL, Chaetomium Kunzeanum Zopf, Pyrenochaeta humicola 

 Oud., Chaetomella horrida Oud., Trichocladium asperum Harz, Stachy- 

 botrys alternans Oud., Sporotrichum bombycinum (Corda) Rabh., Sporo- 

 trickum roseolum Oud. et Beijer., Sporotrichum griseolum Oud., Botrytis 

 vulgaris Fr., Mycogone puccinoides (Preuss.) Sacc, Stemphylium macro- 

 sporoideum (Ben. Br.) Sacc, Cladosporitim herbarum (Pers.) Link, Epicoccum 

 purpurascens Ehren. It is unfortunate, from our point of view, that there 

 is no mention made as to just which species were isolated from the soil 

 and which species from the air. 



The most extensive work as to the number of species isolated from the 

 soil is that of Oudemans and Koning ('02). The isolation was carried out 



('02) Oudemans, C. A. J. A., ct Koning, C. J. Prodrome d'une flore mycologique 

 obtenue par la culture sur gelatine preparee dc la terre humeuse du Spanderswoud 

 pres Bussum. Arch. Neerl. Sci. Nat. scr. 2, 7: 266-298. pis. 41. 1902. 



('04) van Iterson, C. J. Die Zersetzung von Cellulose durch aerobe Mikroorganis- 

 men. Centbl. Bakt. u. Par. Abt. 2, 11: 689-698. pi. i. 1904. 



