3H 



Bulletin 240. 





!™--.\ 



The flasks and bottles were wide mouthed, each received 250 cc. medium, 

 were plugged with cotton and sterilized in the autoclav. 



The seedlings were prepared as follows : radish seed were rinsed in 

 sixty-five per cent alcohol for one minute, then in a one-tenth per cent 

 corrosive sublimate for five minutes, followed by rinsing in four per cent 

 formalin for eight minutes. The rinsing was done in a Petri dish, the 

 formalin was decanted and the 

 seeds were lifted with the aid 

 of a looped, flamed platinum 

 needle and were transplanted on 

 to wet cotton in a test tube pre- 

 viously sterilized in the autoclav. 

 The cotton was slanted in the 

 tube and sufficient water was 

 added to cover the base of the 

 slant (See Fig. 125). The seeds 

 were placed in the test tubes 

 April 6, igo6, and on April 9th 

 young seedlings were trans- 

 planted to the culture flasks. 



\- 







'-S -^i 



*•««::••$«; 



FIG. 124. — Sunflower seedlings growing ill jnnshroom material. Photographed ]\1 ay 

 yth. From left to righ' iliJ material is as follows: Fir^t, distilled water; second, 

 not previously fermented mushroom material; third, stronger concentration of pre- 

 viously fermented mushroom material; fourth, weaker concentration of previously 

 fermented mushroom material. 



The seedlings were lifted with a flamed platinum needle and, as an 

 assistant opened the culture flask by holding it in a nearly horizontal 

 position to avoid as much as possible the gravitation of germs into the 

 medium were transplanted one each in a llask. Where the root was long 

 enough it was pushed into the agar-agar. After a few days the few seed- 

 lings which had not gained a rootliold were ])ushed witli a platinum 

 needle into the agar-agar. The transplanting of the seedlings was done 



