288 ANNUAL REPORT OF THE Off. Doe. 



same day, and all transfers since have been made simultaneously. 

 The comparative jjathogcnicily of these cultures is shown in Tables 

 I and II. 



Cultures H and K were tested also for* puppies and pigs by feed- 

 ing, as described below. 



Culture H was reeovei'ed from the horse, goat, two puppies, two 

 hogs, and man; while culture K was recovered only from two pup- 

 pies and two hogs, the absence of lesions in other animals inoculated 

 making it impossible to obtain a series comparable to culture H. 

 The virulence of these recovered cultures, with the exception of that 

 from the horse, was tested for guinea-pigs and rabbits, the results 

 being given in Table III. 



Human cultures I, J, and W, and bovine cultures F, Q, QQ and 

 T, were isolated as occasion arose, and their virulence tested only 

 for guinea-pigs and rabbits. The dose and the mode of inoculation 

 were uniform in all cases, and the animals were kept under the 

 same condftions, but beyond this the tests are not entirely parallel. 

 The results are given in Table IV. 



In addition to those cultures here mentioned, we have isolated and 

 examined nine others, morphologically and cultuially. These have 

 been taken into account in forming our cooclusions, as well as the 

 very careful descriptions given by Dr. Theobald Smith of the cul- 

 tures studied by him. 



The terms "human" and ^'bovine" have been applied to cultures 

 to denote their origin from man or from cattle. Each culture is 

 designated by a letter of the alphabet, and the generation indicated 

 by a small figure at the lower right corner. Cultures which have 

 been inoculated and recovered retain their original letter, a second 

 letter inclosed in brackets being added to mark the recovered cul- 

 ture. 



2. The second i)art of the work has been the inoculation of ani- 

 mals with tubei-culous material from man and cattle. The plan and 

 methods are described in another section, while the results are given 

 in Tables V and VI. As a part of this experiment, human tubercu- 

 lous sptitum has been fed to two calves, and four have been inocu- 

 lated with the same material. These have been included in Table 

 V, and described farther on. 



