296 ANNUAL, REPORT OF THE Off. Doc. 



and condemned. The lungs were the seat of an extensive and \gd.^ 

 continued tuberculos:ls. Most of the foci had undergo«e caseation. 

 The liver vras involved also, but to a less extent. The gland from 

 which the culture was obtained had undergone cheesj degeneration. 

 On May 17, ISUi), guiuea-pigs were inoculated intraperitoneally with 

 an emulsion made from the centre of this gland. On July G one 

 pig was killed and cultures made on dog's serum. Oo July 28 the 

 first growth was observed, and on August 18 sub-cultures were made 

 from the omentum. (Jrowth was fairly abundant. The bacilli were . 

 short, thick, straight and stained evenly and deeply. 



Culture K (humauj'was isolated from the lung of an adult negro 

 man. The disease was i\ot severe and was confined almost entirely 

 to the lungs. Death was due to heart trouble. Guinea-pigs were 

 inoculated intraperitoneally with an emulsion made from nodules 

 taken from the lungs on June 10, 1899. On June 28, a pig was killed 

 and cultures made on dog's serum. Growth was first observed on 

 July 29, the cultures from the spleen being used for sub-cultures, 

 the first of which was made on August 18. This culture grew rap- 

 idly and abundantly from the begi^ining. The bacilli were quite 

 long, slender and curved. They stained somewhat faintly, and 

 showed a marked tendency to beading. 



COMPARISON OF CULTURES L (BOVINE) AND M (HUMAN). 



History of Cultures. — Culture L (bovine) was obtained from the 

 milk of a cow with tuberculosis of the udder. On May 22, 1899, a 

 guinea-pig was inoculated with 10 c. c. of this milk. It died July 8, 

 and from it a second guinea-pig was inoculated intraperitoneally 

 with an emulsion made from the spleen and omentum. On August 

 25 this pig was killed and two others inoculated intraperitoneally 

 with an emulsion from the spleen and omentum, (^oe of these was 

 killed on September 18, and cultur<>s made on dog's serum. On Oc- 

 tober 20 growth was first observed. On November 17 sub-cultures 

 were made. The gTowth for several generations was very scanty. 

 The bacilli were extremely short, many oval in shape. They took 

 the stain evenly and deeply. 



Culture M (human) was obtained from the sputum of a young adult 

 female. Throat symptoos were most marked in this case. The dis- 

 ease was rapid and violent, death occurring in about five weeks after 

 the sample of sputum was obtained. It contained large numbers 

 of tubercle bacilli. On July 12, 1^99. a guinea-pig was inoculated 

 subcutaneously with the sputum. It was killed August 25, and two 

 pigs inoculated intraperitoneally with an emulsion made from the 

 spleen pnd omentum. On September 18 a pig was killed and cul- 

 tures made on dog's serum. Growth was first observed on October 



