No. 6. DEPARTMENT OF AGRICULTURE, 2'J7 



1~), and sub-cultures were made on October 17. (Jrowth was rapid 

 and abundanl from the lirst, and the lirst transfers made from the 

 original culluic 1(» <^lyceiin agar, grew luxuriantly. The bacilli in 

 the original culture were rather short, irregular in shape, many 

 S-shaped, and as a rule stained quite evenly and deeply. In sub- 

 cultures (he bacilli soon became much longer, straighter and showed 

 beading. 



Swnmary and Postmortern Notes. — It will be seen from the above 

 tables that for each culture ten animals were used. Of the ten inocu- 

 lated with culture H (bovine), nine died, while only one had to be 

 killed, this being the horse; while of those inoculated with culture 

 K (human) only three died, while seven had to be killed. With each 

 of cultures L (bovine) and M (human) eight animals were inoculated. 

 Of those inoculated with culture L, six died and two were killed; 

 while for culture M four died and four were killed. Taking the totals 

 of these four cultures, eighteen animals were used for the bovine cul- 

 tures and eighteen for the human. Of those inoculated with bovine 

 tubercle bacillus, fifteen died and three were killed; while of those 

 inoculated with the human cultures, seven dred and eleven were 

 killed. By making a closer study of the tables, further dilTecences in 

 virulence between the bovine and human cultures may be brought 

 out. All the guinea-pigs inoculated with those four cultures died, 

 the extent of the postmortem lesions being practically identical in 

 all of them. Those inoculated with the human culture K lived al- 

 most ten days longer Ihan those inoculated with the bovine culture 

 H; while those inoculated with human culture M lived seven and one- 

 third days longer than those inoculated with bovine culture L. All 

 the rabbits inoculated with both of the human cultures had to be 

 killed and showed no postmortem lesions whatever, while those 

 inoculated with the bovine cultures died, with extensive lesions. 

 These lesions \aried somewhat in the different animals, necrotic 

 areas at the point of inoculation, and complete involvement of the 

 lungs being common to them all. The kidneys were also generally 

 involved. Only one animal had generalized tuberculosis, involving 

 the lungs, liver, kidneys, spleen and omentum. 



Dogg. — The dogs showed a varying degree of susceptibility to all 

 the cultures. Both of those inoculated with bovine culture H died, 

 one in fifty-nine days, the other in sixtj'-six days. The smaller of 

 the two had shown signs of illness for some days before death. The 

 point of inoculation was well marked in the lung by a mass of tuber- 

 cles 2.5 cm, in diameter. Both lungs were infiltrated throughout 

 with gray nodules from 2 mm. to 4 mm. in diameter. Both pleural 

 cavities contained a considerable amount of purulent effusion. The 

 right pleura was roughened, without adhesions. The outer surface 

 of the pericardium was thickly sown with minute nodules about 2 



