298 ANNUAL REPORT OF THE Off. Doo. 



mm. in diameter, its innerioi' being smooth. The liver was infiltrated 

 throughout and necrotic, giving a nutmeg apijearance. The right 

 kidney contained a number of tubercular nodules; the left was con- 

 gested, but otherwise normal. The spleen had escaped infection. 

 Through an accideut no postmortem was obtained of the other dog, 

 but from his symptous for some days before death it is reasonably 

 certain that the disease was general, allecting particularly, perhaps, 

 the lungs. Both of the dogs inoculated with human culture K had to 

 be killed, the lesions in both being insignificant. In one the only 

 marked change which could be found corresponded to the point of 

 inoculation in the lung, where a small excavation was found almost 

 completely filled in with scar tissue, radiating from the centre in star 

 shape. Scrapings from cut surfaces of this area revealed the pres- 

 ence of tubercle bacilli. All other organs were entirely normal. In 

 the other dog the point of inoculation was marked by a large nodule 

 in the pleural surface of the lung. The surfaces of both lungs showed 

 a great number of minute nodules. Scrapings from cut surfaces re- 

 vealed tubecle bacilli in small numbers. On the external surface of 

 the pericardium was a nodule about one inch in diameter which con- 

 tained calcareous foci, incapsulated in dense fibrous material. The 

 mediastinal glands were enlarged and contained a number of caseous 

 foci. In all other respects the animal seemed normal. 



Horses. — A marked diflerence was noted in the effect of the cul- 

 tures on the horses. The horse inoculated with bovine culture H 

 showed no ill effects from the injection for some months after the in- 

 jection. In the latter part of March, nearly four months after the 

 injection, some emaciation could be noted, and the respiration was 

 quickened, being on an average of twenty-two a minute and some- 

 what labored. On the first of April the temperature averaged 102.2 

 degrees Fahrenheit. The symptoms grew very slightly more marked 

 and on June 25, six months and a half after inoculation, the animal 

 was killed. It had lost forty pounds in weight, though its condi- 

 tion was still fair. The costal pleurae were extensively covered with 

 masses of nodules of from 2 mm. to 18 mm. in diameter, averaging 

 nearly 12 mm. in thickness. These growths were somewhat more 

 extensive on the left side than on the right, and tended to be more 

 isolated and somewhat larger in size. The lungs were a grayish 

 pink, the blood-vessels being plainly outlined in a delicate network 

 over the surface. They were covered with nodules, varying from 

 1 mm. up to 6 mm. of an inch in size, and closely packed. Both lungs 

 were hard, dense and devoid of elasticity, having much the feel of 

 liver. At the point of inoculation on the right lung a small depres- 

 sion was found, filled in with a star-shaped cicatrix. This cicatricial 

 tissue was surrounded with a nodule about 2.5 cm. in diameter, con- 

 taining cheesy areas and much fibrous tissue. lu the posterior part 



