No 6. DEPARTMENT OF AGRICULTURE. 305 



Cf'MPARISON OF OTHER HUMAN AND liOVlNK CULTURES. 



HISTORY OF CULTURES. 



Culture F {Bovine). — Obtaiued from a large tninor on the dia- 

 phragm of a cow svhicli had been slaughtered for beef. The tumor 

 was 13 cm. long and from 7 to 9 cm. in diameter. On the surface 

 were a number of grape-like masses of pearly lustre. On section 

 numerous caseous areas were found, from which an emulsion was 

 made. It contained many tubercle bacilli. A guinea-pig was in- 

 oculated intraperitoneally May IG, 1899. Death occurred on June 

 8, and from its tissues a second pig was inoculated intraperitoneally. 

 This pig was chloroformed July 31, and a third pig inoculated in the 

 same manner, which was chloroformed August 21, and cultures made 

 from si)leen and omentum. Scanty growth was observed on Sep- 

 tember 27. Under the microscope the bacilli were thick, short and 

 showed little tendency to beading, staining deeply and uniformly. 

 The further history of this culture is given in the paragraph on 

 "Retention of Ghaiacteristics in Culture." 



Culture Q {Bovine). — Obtained from the lungs of cow No. 598G. 

 This cow was brought to our department on July G, 1898, for ex- 

 perimental purposes, being considered then an advanced case of 

 tuberculosis. She was emaciated and coughing badly. Many tuber- 

 cle bacilli were found in the mucus she coughed up, which were long, 

 thin and much beaded. She died February 12, 1900. The lungs 

 were infiltrated throughout, many of the areas having undergone 

 caseation and calcification. It was diflficult to understand how the 

 animal lived as long as it did. The mediastinal glands formed a 

 tumor 35 cm. long by 15 cm. in diameter, which was cheesy on sec- 

 tion. The mesenteric glands were enlarged and cheesy. A guinea- 

 pig was inoculated in the peritoneal cavity on November 15, 1889. 

 On November 30 the animal was killed and cultures made. Growth 

 was observed first on January 10, and sub-cultures made on the same 

 date. Growth was always scanty. 



Culture QQ {Bovine). — Was obtained from the mesenteric gland 

 of cow No. 598G, An emulsion from the centre of the gland was in- 

 oculated into the peritoneal cavity of a guinea-pig February 13, 1900. 

 On March 5 it was chloroformed and cultures made. Growth was 

 observed on April 12, and sub-cultures were made on April 17. 

 Growth was more abundant than that on the preceding culture (Q), 

 but still scanty. 



Culture T{Bovine. ) — Was obtained from milk sent in for examina- 

 tion by a dairyman in Philadelphia. It was inoculated into the 

 peritoneal (avity of a guinea-pig January 24, 1900. The guinea-pig 

 20^6—1901 



