306 ANNUAL REPORT OF THE Off. Doc. 



was chloroformed on April G. With an emulsion of its spleeo two 

 other guinea-pigs were inoculated intraperitoneal!}' on the same 

 date. One of these was chloroformed on April 28, and cultures made. 

 Growth was observed first on June 23, and cultures made the same 

 dav. Growth was abundant and luxuriant. 



Culture TJ {Human). — Obtained from a child three years of age, 

 whose death was due to tubercular meningitis. Autopsy. Lungs 

 and bronchial lymphatic glands full of miliary tubercles. Anterior 

 mediastinal glands much enlarged. Spleen extensively involved, 

 liver less so. On abdominal surface of diaphragm were a number of 

 flat, yellow nodules. Mesenteric glands enlarged, and contained old, 

 yellow, cheesy nodules. One small tubercle found in right supra- 

 renal. Purulent and cheesy nodules found in meninges and encep- 

 halon. The culture was obtained from mesenteric glands. 



The pathologist, Dr. Hand was uncertain as to the origin of this 

 case. 



Guinea-pigs were inoculated intraperitoneally on June 15, 1900. 

 One was killed on July 23, and with its tissues a second was inocu- 

 lated intraperitoneally. This was chlorformed on September 12, and 

 cultures made. On October 11, no growth having taken place, the 

 blocks of tissue were transferred to fresh serum and stirred. On 

 December 4 growth was first observed, and on December 15 sub-cul- 

 tures were made. Growth has been rapid and abundant. 



Culture W {Human). — Obtained from a child nine months of age, 

 of scrofulous parentage. The cervical glands on both sides were en- 

 larged. Autops.y. A double broncho-pneumonia was found. One 

 small tubercular nodule was found on surface of left lung, several 

 at apex of right lung. Bronchial glands not enlarged. Tonsils nor- 

 mal in size, but glands on each side of pharynx extending in front of 

 and behind sternomastoid muscle were enlarged, tuberculous, and 

 several contained creamy pus. Three mesenteric glands were en- 

 larged and chet3y; an ulcer was found in the jejunum and another 

 at the ileocecal valve. Peyer's patches and the lymphatic glands 

 about cecum were enlarged. Spleen and liver contained tubercles. 

 The mesenteric glands were employed for the isolation of the culture. 



This case was considered by Dr. Hand as being probably of ton- 

 sillar or pharyngeal origin. 



Guinea-pigs were inoculated intraperitoneally on October 5, 1900. 

 From one which died on January 4, 1901, a second was inoculated in- 

 traperitoneally. This was killed March 4, and cultures made. 

 Growth was first observed on April 12, and sub-cultures made the 

 following day. 



Culture BB {Human). — Obtained from a child seventeen months of 

 age. Death was due to tubercular meningitis. Autopsy. Lungs 



