No. 6. DEPARTMENT OF AGRICULTURE. 307 



normal, except postciioi* and part of rij^lit upper lobe, which was 

 consolidated, red on section, and Lad excess of connective tissue. 

 Bronchial aud mediastinal glands not enlarged. Spleen, liver and 

 kidneys show pearly tubercles. Two feet from lower end of ileum 

 is an ulcer 1 by 2 cm., the long diameter transverse to axis of gut. 

 Nodules are seen on peritoneal surface. Four small ulcers, two 

 above and tw'O below, are found in ileum. Mesenteric glands en- 

 larged, cheesy and some purulent. Meninges show yellow tubercles 

 most marked along longitudinal fissure, in choroid plexus, and over 

 cerebellum. The mesenteric glands were used in obtaining the cul- 

 ture. 



This case is considered by Dr. Hand the clearest one of primary 

 intestinal tuberculosis ever seen by him. 



Guinea-pigs were inoculated intraperitoneally with an emulsion 

 made from the mesenteric glands on March 9, 1901. One was chloro- 

 formed on April 19 and cultures made. Scanty growth on one tube 

 was found June, and sub-cultures made. 



The results given in Table IV show for all the bovine cultures a 

 greater virulence than for any of the human cultures. The average 

 life of the guinea-pigs inoculated with human bacilli was more than 

 twice that of those inoculated with bovine. All the rabbits which 

 received human cultures gained in weight and had finally to be killed, 

 while for the bovine they died, and presented postmortem extensive 

 lesions, the lungs and kidneys being the chief seats of the disease 

 in all animals. 



PART 11. 



TESTING THE PATHOGENIC POWER OF TUBERCULOUS MA- 

 TERIAL OF HUMAN AND BOVINE ORIGIN. 



In addition to the study of a number of pure cultures obtained 

 from man and cattle, we have examined the pathogenic power of 

 tuberculous material from the two sources also, making a series 

 of inoculations which are as nearly parallel as possible. The results 

 of these tests are shown in Tables V and VL The plan was to in- 

 oculate a series of animals with tuberculous tissues rubbed into a 

 smooth suspension, and at the same time to inoculate a number of 

 guinea-pigs with the same, which on their death would furnish ma- 

 terial for another series of inoculations parallel with those made di- 

 rectly from the human and bovine tissues. It was believed that 

 the material from guinea-pigs would more nearly represent pure cul- 

 tures of the respective organisms than would be obtained from the 



