No. 6. ^ DEPARTMENT OF AGRICULTURE. 3i:i 



way, lUid used for the iuoeulatioii of a second series of auimals, as 

 seeo iu Table V. 



Sources of Haman Tiiberculous Material. — 'JMiis material was ob- 

 tained from three bodies, the lungs only being used. Two of the 

 three were cases of well-marked acute miliary tuberculosis, the other 

 a case of chionic phthisis, all adults. The material was prepared 

 for injection in the same way as described for the bovine, the sus- 

 pension being made as nearly of the same thickness as possible. The 

 (irst series of animals was inoculated with the tissues from one of 

 the cases of acute miliary tuberculosis on August 27. A number of 

 guinea pigs were also inoculated with the object of having their 

 tissues for a second series of inocuhitions, as with the bovine ma- 

 terial, but they all died of septicemia within forty-eight hours, pre- 

 venting the execution of this plan. With the exception of the pigs, 

 and the calves inoculated with sputum, the other animals were in- 

 oculated from the two cases mentioned above. 



Method of Inoculation. — In all the animals the iojectioo was made 

 into the lung, the field of puncture being shaven, washed with soap 

 and water, next with alcohol and ether, and lastly with a 1:1000 

 solution of bifhlorid of mercury. The site of puncture was, for the 

 horses, between the eighth and ninth ribs; for the pigs, sheep and 

 dogs, bctweeti the sixth and seventh ribs; and for the cats, between 

 the fifth and sixth ribs. 



^ . CONDENSED POSTMORTEM NOTES. 



Horses. Bovine Material. Tv)0 Animals. — One died after fifty- 

 four days, with loss of 105 pounds; the other was killed after sixty- 

 six days, having lost sixty pounds. In both the desease was maioly 

 in thoracic cavity. In the one inoculated with material direct from 

 cow, both lungs were involved throughout. In the other the tuber- 

 culous process w^as confined to the lung into which injection was 

 m.nde, and marked only «ieai' site of inoculation. 



Ilovses. Human Material. Ttvo Animals. — Both had to be killed. 

 One was entirely normal, not even the point of inoculation being 

 discernible. In the other the tuberculous process was confined to an 

 area 5 em. in diameter, with the point of inoculation as a centre. 



Pigs. Bovine Material. Four Animals. — All died with an aver- 

 age length of life of fifty and three-quarter days. The tw^o which 

 received the bovine material direct lived sixty-eight and one-half 

 days, as against thirty-three days for those inoculated from the 

 gninea-})igs. In all, the lungs were tbe chief seat of the disease, all 

 showing an acute miliary tuberculosis of both organs. In the two 

 inoculated directly from the cow, the abdominal cavity was invaded 

 to a limited extent, seen only in the mesenteric glands. 



Pigs. Human Material. Six Animals^ — Five died and one was 

 killed. The average length of life of the two inoculated directly 



