No. 6. DEPARTMENT OF AGRICULTURE. 315 



scess cavity 7 c. in. long by 2. 5 inm, in diameter. The abdominal 

 organs were not involved. 



Cats. Bovine Material. Two Animals. — Both became rapidly ill, 

 one dying in twenty and the other in thirty-one days. In both the 

 lungs were the seat of an acute miliary tuberculosis. lo the one 

 which received the bovine material direct, the spleen contained a 

 number ol cheesy areas. In the other the mesenteric glands were 

 enlarged and cheesy. The more rapid progress of the disease in the 

 latter was probably due to its smaller size and the larger dose. 



Cats. Human Material. Two Animals. — One cat died after two 

 days and has not therefore been included in the averages. The 

 second was killed after 19^ days and found to be normal throughout. 

 Not even the point of inoculation could be determined. 



Summary. — Twelve animals were inoculated with bovine material. 

 Ten of these died, while two survived — the horse and the dog which 

 received the tissues of the guinea-pig. 



Fourteen animals were inoculated with the human material, of 

 which eight died and six were killed. 



For most of the animals the tuberculous material direct from the 

 cow seemed to be the more virulent. The sheep and pigs were ex- 

 ceptions to this, the pigs notably so, those receiving the tissues of 

 guinea-pigs dying in thirty-three days, as against sixty-eight and 

 one-half days for the others. The averages of life after inoculation 

 given in the table, as well as the greater mortality, show strikingly 

 the increased potency of the bovine material over that from man. 



This difference is further brought out by the extent and character 

 of the lesions produced by the one and the other, as shown in the 

 postmortem notes, and in Table VI. 



Infection of Calves with Human Tuberculous Sputum,. — The re- 

 sults of this experiment are included in Table V, the details which 

 are of considerable interest being supplied here. 



Calf 5984, age five weeks, weight lOS pounds, was inoculated in- 

 traperitoneally with 10 c. c. of sputum from an advanced case of 

 tuberculosis at the University Hospital, on July 29, 1S98. The 

 patient from whom the sputum was obtained was a young adult 

 who had been sick about a year and a half, and had been expectora- 

 ting freely for one year. A cavity had been observed in the left lung 

 about eight months before the sputum was obtained. Death oc- 

 curred soon after. Beyond some slight elevation of temperature 

 this calf showed no effect whatever from the inoculation. On No- 

 vember 3, 1898, it was tested with tuberculin, but gave no reaction. 

 When it was killed on January 14, 1899, it weighed 258 pounds, an 

 increase of 150 pounds. It was in good condition and showed no 

 tuberculous lesions in any part of the body. The site of inoculation 

 could not be determined. 

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