318 ANNUAL REPORT OF THE Off. Doc. 



sputum from an early case of pulmonary tuberculosis at the Univer- 

 sity Hospital. The sputum contained a large number of tubercle 

 bacilli. Soon after inoculation the temperature of the calf rose and 

 continued high, with some remissions, until it was killed. Its ap- 

 pearance was bad, the coat dry and rough, the respiration rapid. 

 It was tested with tuberculin, but the temperature was too high for 

 results. It was killed on August 1, weighiog 190 pounds. On the 

 surface of both lungs there was a slight deposit of fibrin, and on sec- 

 tion a number of hemorrhagic areas were observed in both. The 

 mediastinal and bronchial glands were enlarged and congested. 

 The abdominal cavity contained about twelve ounces of bloody 

 serum. The peritoneum was thickl}^ studded over its entire surface 

 with oodules from 1 mm. to 12 mm. in dimeter, fibrous in character. 

 In many places these nodules had massed together, forming tumors, 

 some 5 cm. in diameter, which were dense and fibrous. The spleen 

 contained many nodules, both on the surface and throughout its sub- 

 stance. The whole omentum was thickly studded with nodules from 

 2 mm. to 12 mm. in diameter, and besides which there were three 

 large masses, dense and fibrous in character, two of which were 15 

 cm. long by 7 cm. wide, and 12 mm. thick; and the third 7 cm. long, 

 by 6 cm, wide, by 4 cm. thick. The abdominal surface of the dia- 

 phragm was thickly studded with nodules, fibrous in character. 

 The mesenterj' was thickened and contained many nodules of small 

 size. The appearance was that of a typical case of grape or pearl 

 disease. The mesenteric and mediastinal glands were enlarged and 

 somewhat caseous. Twenty c. c. of an emulsion made from these 

 glands, which contained a large number of tubercle bacilli, were in- 

 jected on August 2 into the peritoneal cavity of calf 9810, four weeks 

 old, weight 132 pounds. The animal showed no ill effects whatever 

 from inoculation and was killed oo January 10, 1899. A careful 

 postmortem examination showed it to be normal in all respects. 



Simimary. — Four calves of nearly the same age received intra- 

 peritoneally 10 c, c. of human tuberculous sputum from different 

 sources, but in all cases containing a large number of tubercle bacilli. 



One showed no ill effect from the injection execept a slight rise 

 of temperature, and wheti killed the autopsy was entirely negative. 



Of the other three, two had persistent high temperature follow- 

 ing the injection, but only one showed marked illness otherwise. 

 Postmortem examination proved that all had become infected with 

 tuberculosis, the lesions in two being quite extensive. From each 

 of these two a second calf was inoculated intraperitoneally with an 

 emulsion made from well-developed iiodules. In both cases the 

 emulsion was rich in tuber(;le bacilli, and a large dose (20 c. c.) was 

 injected. The result was absolutely negative in one animal and prac- 

 tioally 80 in the other. 



Since both calves received a much larger number of tubercle 



