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  connection 
  between 
  the 
  cambium 
  layers 
  of 
  host 
  and 
  of 
  guest. 
  In 
  

   southern 
  regions 
  of 
  the 
  United 
  States 
  the 
  union 
  occurs 
  much 
  more 
  

   readily 
  than 
  in 
  the 
  north. 
  My 
  experiments 
  have 
  been 
  made 
  chiefly 
  

   with 
  reference 
  to 
  developing 
  methods 
  of 
  propagating 
  nut 
  trees 
  in 
  

   the 
  north. 
  All 
  of 
  the 
  usual 
  methods 
  common 
  among 
  nurserymen 
  

   have 
  been 
  practically 
  failures, 
  but 
  certain 
  unusual 
  methods 
  seem 
  to 
  

   promise 
  success. 
  

  

  One 
  unusual 
  method 
  which 
  was 
  suggested 
  at 
  last 
  year's 
  meeting 
  

   by 
  our 
  member 
  Mr. 
  J. 
  F. 
  Jones, 
  has 
  given 
  a 
  good 
  proportion 
  of 
  

   catches. 
  This 
  consists 
  in 
  using 
  wood 
  which 
  is 
  more 
  than 
  one 
  year 
  

   old 
  for 
  scions. 
  Some 
  of 
  the 
  scions 
  of 
  shagbark 
  hickory 
  from 
  wood 
  

   four, 
  five, 
  and 
  even 
  six 
  years 
  of 
  age 
  have 
  caught. 
  The 
  chief 
  diffi- 
  

   culty 
  has 
  consisted 
  in 
  starting 
  the 
  buds 
  of 
  this 
  old 
  wood 
  (latent 
  

   buds) 
  before 
  vigorous 
  sprouts 
  from 
  the 
  stock 
  diverted 
  all 
  the 
  sap. 
  

   It 
  has 
  been 
  necessary 
  to 
  give 
  much 
  attention 
  to 
  the 
  removal 
  of 
  these 
  

   vigorous 
  stock 
  sprouts. 
  I 
  seem 
  to 
  have 
  made 
  the 
  observation 
  that 
  

   if 
  a 
  small 
  side 
  branch 
  from 
  old 
  wood 
  carries 
  a 
  large 
  terminal 
  bud, 
  

   this 
  bud 
  will 
  start 
  promptly 
  when 
  old 
  wood 
  constitutes 
  the 
  rest 
  of 
  

   the 
  scion. 
  

  

  A 
  method 
  which 
  I 
  employed 
  for 
  the 
  first 
  time 
  this 
  year, 
  which 
  

   appears 
  to 
  have 
  resulted 
  in 
  securing 
  union 
  between 
  stock 
  and 
  scion, 
  

   has 
  been 
  employed 
  between 
  different 
  species 
  of 
  hickory 
  trees. 
  It 
  

   belongs 
  among 
  the 
  inarching 
  methods 
  in 
  classification. 
  It 
  seemed 
  

   probable 
  that 
  if 
  a 
  scion 
  were 
  to 
  be 
  supplied 
  with 
  sufficient 
  water 
  to 
  

   prevent 
  drying 
  out, 
  in 
  advance 
  of 
  granulation-cell 
  connection, 
  we 
  

   might 
  meet 
  with 
  success. 
  

  

  The 
  first 
  line 
  of 
  experimentation 
  with 
  this 
  idea 
  in 
  mind 
  was 
  con- 
  

   ducted 
  last 
  year. 
  The 
  scion 
  when 
  grafted 
  upon 
  the 
  stock 
  was 
  

   deprived 
  of 
  its 
  top 
  bud, 
  and 
  a 
  smaU 
  test 
  tube 
  filled 
  with 
  water 
  and 
  

   fitted 
  with 
  a 
  rubber 
  cap 
  was 
  adjusted 
  over 
  the 
  site 
  previously 
  occu- 
  

   pied 
  by 
  the 
  top 
  bud. 
  This 
  in 
  practical 
  working 
  really 
  did 
  keep 
  the 
  

   cells 
  of 
  the 
  scion 
  alive 
  and 
  in 
  good 
  condition 
  for 
  a 
  long 
  time, 
  but 
  

   there 
  was 
  always 
  a 
  tendency 
  for 
  the 
  water 
  to 
  become 
  impure 
  because 
  

   of 
  the 
  growth 
  of 
  various 
  algae 
  and 
  other 
  microbes. 
  Evidently 
  

   the 
  water 
  when 
  used 
  in 
  this 
  way 
  helped 
  to 
  furnish 
  a 
  balance 
  be- 
  

   tween 
  the 
  negative 
  and 
  the 
  positive 
  sap 
  pressures 
  which 
  occur 
  under 
  

   changing 
  conditions 
  of 
  barometer 
  and 
  temperature, 
  and 
  which 
  are 
  

   influential 
  in 
  the 
  matter 
  of 
  cellular 
  repair. 
  The 
  introduction 
  of 
  

   germicides 
  into 
  the 
  water 
  of 
  the 
  test 
  tube 
  prevented 
  the 
  develop- 
  

   ment 
  of 
  adventitious 
  organic 
  life, 
  but 
  at 
  the 
  same 
  time 
  seemed 
  to 
  

   interfere 
  with 
  normal 
  cell 
  activity 
  at 
  the 
  junction 
  of 
  stock 
  and 
  

   scion. 
  

  

  