66 



fection and the little area of green wood connecting the two points 

 is thickening. The tree looks healthy otherwise, with no signs of 

 sprouts coming from the root which is one of the early evidences of 

 infection sufficient to close off the circulation. 



October 26th. No evidence of further invasion and tree looks fine, 

 although the injury of course is not fully healed over. There is no, 

 extension of blight into the callus. 'The tree is dormant. These 

 three injections apparently protected this native American chestnut 

 pretty well. 



June, 1926. Tree looks thrifty and apparently recovered. Sawed off 

 top and grafted. 



July 3. Grafts died off and examination showed blight had reinvaded 

 the callus and killed the tree. 



September 8, 1926. Old stock dead. Four healthy new sprouts on 

 its roots. 

 No. 103 



June 26, 1925. Height four and one-half feet, 5-8 inch in diameter. 

 Tree a sprout from a blighted stump but apparently healthy. Given 

 three injections and on Aug. 10th innoculated with a piece of blight- 

 ed bark injected under the bark and waxed. Area became infected 

 and went almost around the tree, although the infection did not 

 seem very virulent, for there are areas of live cambium in the in- 

 fection, which has now become apparently inactive. This tree, like 

 102, is apparently not sufficiently protected by three injections, or 

 sufficient time had not been allowed before the innoculation was 

 made. 



May 25, 1926. Top blighted and died at area of innoculation. Graft- 

 ed with Fuller and stump cut off four inches above ground. 



September 8th. Graft doing nicely and no further evidence of blight. 

 No. 106 



July 15, 1925. Japanese chestnut 45332 from U. S. Dept. Agr. Tree 

 six feet high. Tree showed large area of blight at first crotch and 

 on a couple of small limbs above site of first injection. This is the 

 last of several Japanese trees to blight and each time killing the 

 tree to the ground in its turn, the roots sprouting again the following 

 year. 



July 24. Large area seems to be walling off and bark separating. A 



