68 



circulation is shut off. The leaves are quite yellow and the tree 

 looks bad. 



August 3()th. Callus nearly if not quite together. I took my knife 

 and cut the edges of the callus just a little thinking it might heal 

 together easier. 



September 30th. Callus has united, having jumped the gap and taken 

 up the circulation nearly half way around the tree. The leaves are 

 again green and the tree has a good appearance, which shows that 

 it has again taken up the normal function. 



October 26th. The tree seems apparently entirely recovered and 

 healthy, although the denuded area has not entirely healed over. 

 There is no evidence of further blight, and no more callus being 

 thrown out. It has evidently become dormant for the winter. Evi- 

 dently Morris No. 1 has a good deal of immunity, for this one dosc 

 has apparently not only cured it but protected it from further in- 

 vasion up to this time. I have not seen any native American chest- 

 nut show anything like this amount of resistance following the in- 

 jection of one dose, up to this time. 



September 8, 1926. Tree apparently healthy, leaves of good color, 

 made a nice growth this summer, and the old scar is almost healed 

 over. 



Apparently, from the above, it is possible to inject blighted trees 

 of the chinquapin, chinquapin x chestnut hybrids, Japanese, and 

 Chinese chestnuts and have them recover, but with the exception of a 

 few cases, the American chestnut that is blighted at the crown will 

 die off before enough ferment can be stimulated to protect it. How- 

 ever, in a few cases, especially if injected early in the spring, they may 

 survive. I had some that had every appearance of recovering until I 

 sawed the top off and grafted them. Then they were attacked with re- 

 newed vigor and died promptly. After a few injections it is altogether 

 probable that the American chestnut will perform similar to other 

 chestnuts that have a certain amount of resistance, like the Japanese 

 or chinquapin. 



Amount Necessary to Protect 



One dose has apparently protected the Morris No. 1 hybrid. 



Two have protected a Japanese. 



One, two and three have not protected an .American, although No. 



