110 ANNUAL REPORT OF THE Off. Doc. 



Thiid Series. — Infection hy Siibcutaneous Inoculation. — Seven ani- 

 Dials were inoculated. For three animals the material used was ob- 

 tained from funf:;us masses from the joints in cases of white swelling; 

 for three others from acute miliary tuberculoe^is, and in one from the 

 lung of a horse wlliich had developed a marked pulmonary tuberculo- 

 sis due to the intravenous injection of material from the lung of a 

 man. In all cases a tumor developed at the point of inoculation, 

 which Chauveau diescribes as being a tuberculous tumor altogether 

 typical. The neighboring glands showed always more or less in- 

 volvement, but in no cases was there any general infection. 



I have quoted these experiments at some length for the reason 

 that they seem often to be overlooked, and for the further reason 

 that they have recently been directly misquoted. Indeed, Koch him- 

 self said in his address before the British Congress on Tuberculosis, 

 "If one studies the older literature of the subject, and collects the 

 reports of the numerous experiments that were made in former times 

 by Chauveau, Gunther and Harms, Bollinger, and others, wftio fed 

 calves, swine, and goats with tubercular material, one finds that the 

 animals that were fed with the milk and pieces of the lungs of tuber- 

 cular cattle always fell ill of tuberculosis, whereas those that re- 

 ceived human material with their food did not." 



Bollinger, in 1879, inoculated a young calf in the peritoneal cavity 

 with material from a human lung. When killed after seven months 

 the mesentery and peritoneal covering of the spleen presented a num- 

 ber of tumors from the size of a pea to that of a walnut, and which 

 microscopically were identical with those found in pearl disease 

 under natural conditions. The retroperitoneal and mesenteric glands 

 were tuberculous also. 



Klebs caused a tuberculosis of the peritoneum identical with that 

 which is seen under natural conditions by the intraperitoneal injec- 

 tion of human tubercular material. Kitt, also, is quoted by Pro- 

 fessor Johne as Qiaving had a similar result accompanied with gen- 

 eralized lesions following the intraperitoneal injection of the juice 

 from a scrofulous gland taken from the neck of a man. 



Crookshank injected tuberculous sputum into the peritoneal cav- 

 ity of a calf, causing death on the forty-second day. The autopsy re- 

 vealed extensive disease. "Extending over the mesentery from the 

 point (inoculation) there were hundredis> of wart-like, fleshy new- 

 growths, some quite irregular in form, others spherical or button- 

 shaped. There were similar deposits on the under surface of the 

 liver, on the spleen, in the gastrosplenic omentum, and on the peri- 

 toneal surface of the diaphragm. On microscopic examination ex- 

 tremely minute tubercles were found disseminated throughout the 

 lungs and liver. Tubercle bacilli were found in these organs and' 

 in the peritoneal deposits," 



